Analysis of protein interactions using fluorescence technologies

被引：168

作者：

Yan, YL ^{[1
]}

Marriott, G ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Physiol, Madison, WI 53706 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2003年 / 7卷 / 05期

关键词：

D O I：

10.1016/j.cbpa.2003.08.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Biophotonics techniques, especially those involving fluorescence, are widely used in proteomics to characterize the in vitro interactions between proteins in high-throughput mode. On the other hand, fluorescence-based imaging studies often show that protein activity is regulated through large protein complexes that transiently form at specific sites in the cell. One could therefore argue that a systematic functional analysis of the human proteome requires technologies that are capable of time and spatially resolved, multiplexed analysis of protein interactions within cells.

引用

页码：635 / 640

页数：6

共 31 条

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