Influence of culture conditions on exopolysaccharide production by Lactobacillus rhamnosus strain C83

被引:42
作者
Gamar-Nourani, L [1 ]
Blondeau, K [1 ]
Simonet, JW [1 ]
机构
[1] Univ Paris 11, Inst Genet & Microbiol, Lab Genet Mol Bacteries Interet Ind, CNRS,URA 2225, F-91405 Orsay, France
关键词
D O I
10.1111/j.1365-2672.1998.00574.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The influence of culture conditions on growth and exopolysaccharide (EPS) production bq Lactobacillus rhamnosus strain C83 was investigated using fermenter batch cultures. A temperature shift (from 37 to 25 degrees C) at the beginning of the exponential growth phase (0-5 h) enhanced EPS production. Furthermore, an optimal environmental pH of 6.2-7.2 improved the performance of strain C83 and partially anaerobic culture conditions (pO(2) from 0 to 10%) triggered EPS over-production bq the cells. The sugar composition of this polymer was independent of culture conditions, such as carbon source, medium composition, temperature, pH, pO(2) and growth phases. In all cases, galactose and glucose were the principal components.
引用
收藏
页码:664 / 672
页数:9
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