Determinants of the human infant intestinal microbiota after the introduction of first complementary foods in infant samples from five European centres

被引:259
作者
Fallani, Matteo [2 ]
Amarri, Sergio [3 ,4 ]
Uusijarvi, Agneta [5 ]
Adam, Ruediger [6 ]
Khanna, Sheila [1 ]
Aguilera, Marga [2 ,7 ]
Gil, Angel [7 ]
Vieites, Jose M. [7 ]
Norin, Elisabeth [8 ]
Young, David [9 ]
Scott, Jane A. [10 ]
Dore, Joel [2 ]
Edwards, Christine A. [1 ]
机构
[1] Univ Glasgow, Yorkhill Hosp, Div Dev Med, Human Nutr Sect, Glasgow, Lanark, Scotland
[2] INRA, Unite Ecol & Physiol Syst Digestif, Jouy En Josas, France
[3] Azienda Osped Santa Maria Nuova, Dept Paediat, Reggio Emilia, Italy
[4] Univ Modena & Reggio Emilia, Dept Paediat, Modena, Italy
[5] Karolinska Inst, Sachs Childrens Hosp, Sodersjukhuset, Dept Clin Sci & Educ, Stockholm, Sweden
[6] Univ Med Ctr, Dept Paediat & Adolescent Med, Mannheim, Germany
[7] Univ Granada, Dept Biochem & Mol Biol, E-18071 Granada, Spain
[8] Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden
[9] Univ Strathclyde, Dept Stat & Modelling Sci, Glasgow, Lanark, Scotland
[10] Flinders Univ S Australia, Sch Med, Adelaide, SA 5001, Australia
来源
MICROBIOLOGY-SGM | 2011年 / 157卷
关键词
RIBOSOMAL-RNA PROBES; FORMULA-FED INFANTS; FECAL MICROFLORA; EXOCRINE PANCREAS; SWEDISH INFANTS; FLOW-CYTOMETRY; HUMAN FECES; HYBRIDIZATION; DELIVERY; DESIGN;
D O I
10.1099/mic.0.042143-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Although it is well established that early infant feeding has a major influence on the establishment of the gut microbiota, very little is understood about how the introduction of first solid food influences the colonization process. This study aimed to determine the impact of weaning on the faecal microbiota composition of infants from five European countries (Sweden, Scotland, Germany, Italy and Spain) which have different lifestyle characteristics and infant feeding practices. Faecal samples were collected from 605 infants approximately 4 weeks after the introduction of first solid foods and the results were compared with the same infants before weaning (6 weeks of age) to investigate the association with determining factors such as geographical origin, mode of delivery, previous feeding method and age of weaning. Samples were analysed by fluorescence in situ hybridization and flow cytometry using a panel of 10 rRNA targeted group- and species-specific oligonucleotide probes. The genus Bifidobacterium (36.5 % average proportion of total detectable bacteria), Clostridium coccoides group (1 4 %) and Bacteroides (13.6%) were predominant after weaning. Similar to pre-weaning, northern European countries were associated with a higher proportion of bifidobacteria in the infant gut microbiota while higher levels of Bacteroides and lactobacilli characterized southern European countries. As before weaning, the initial feeding method influenced the Clostridium leptum group and Clostridium difficile+Clostridium perfringens species, and bifidobacteria still dominated the faeces of initially breast-fed infants. Formula-fed babies presented significantly higher proportions of Bacteroides and the C. coccoides group. The mode of birth influenced changes in the proportions of bacteroides and atopobium. Although there were significant differences in the mean weaning age between countries, this was not related to the populations of bifidobacteria or bacteroides. Thus, although the faecal microbiota of infants after first complementary foods was different to that before weaning commenced, many of the initial influences on microbiota composition were still evident.
引用
收藏
页码:1385 / 1392
页数:8
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