Structural organization and chromosomal localization of the human hepatocyte growth factor activator gene - Phylogenetic and functional relationship with blood coagulation factor XII, urokinase, and tissue-type plasminogen activator

被引:28
作者
Miyazawa, K [1 ]
Wang, YM
Minoshima, S
Shimizu, N
Kitamura, N
机构
[1] Tokyo Inst Technol, Fac Biosci & Biotechnol, Dept Life Sci, Midori Ku, Yokohama, Kanagawa 226, Japan
[2] Keio Univ, Sch Med, Dept Mol Biol, Tokyo, Japan
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1998年 / 258卷 / 02期
关键词
hepatocyte growth factor activator; blood coagulation factor XII; gene duplication; genomic organization; chromosomal localization;
D O I
10.1046/j.1432-1327.1998.2580355.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The organization and structure of the gene coding for hepatocyte growth factor activator (HGFA) have been determined by isolation of unique clones from a human genomic library. These clones were characterized by restriction mapping, Southern blotting and DNA sequencing. The complete sequence of the gene was determined and found to span about 7.5 kilobases of DNA and consist of 14 exons separated by 13 introns. The coding region of HGFA consists of multiple putative domains that are homologous to those observed in blood coagulation factor XII (FXII). These regions were found as separate exons in the gene, and the exon/intron arrangement was similar to that of FXII, suggesting that the genes for HGFA and FXII have arisen through gene duplication events from a common ancestral gene. The major transcription initiation site is located 75 bp upstream of the translational start codon. The gene was mapped to chromosome 4p16, using spot-blot hybridization on sorted chromosomes and fluorescence in situ hybridization on metaphase chromosome spreads. The phylogenetic and functional relationships between HGFA and FXII as well as urokinase and tissue-type plasminogen activator are discussed.
引用
收藏
页码:355 / 361
页数:7
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