A new microcatheter enables microcalorimetric assessment of thermogenesis after stimulation in cell cultures

被引：6

作者：

Bottcher, H ^{[1
]}

Furst, P ^{[1
]}

机构：

[1] UNIV HOHENHEIM,INST BIOL CHEM & NUTR,D-70593 STUTTGART,GERMANY

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1996年 / 32卷 / 03期

关键词：

microcalorimeter; stimulation of thermogenesis; thermogenic agent; hormone; fat cell culture;

D O I：

10.1016/0165-022X(96)00010-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new, inexpensive technique facilitates the observation of long-lasting effects (>2 h) of thermogenic agents on cell cultures. A special microcatheter enables addition of hormones or pharmacologic agents to biological samples during microcalorimetric analysis using a ThermoMetric ThermalActivityMonitor without removing the sample ampoules from the measuring position. Technical modification of the calorimeter is not required. Baseline stability with the connected catheter is +/-0.2 mu W over 10 h. The error of the method of catecholamine induced thermogenesis in white adipocytes is 13.4% (C.V.).

引用

页码：191 / 194

页数：4

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