The UP element of the promoter for the flagellin gene, hag, stimulates transcription from both SigD- and SigA-dependent promoters in Bacillus subtilis

被引：14

作者：

Caramori, T ^{[1
]}

Galizzi, A ^{[1
]}

机构：

[1] Univ Pavia, Dipartimento Genet & Microbiol, I-27100 Pavia, Italy

来源：

MOLECULAR AND GENERAL GENETICS | 1998年 / 258卷 / 04期

关键词：

UP element; transcription; promoter; Bacillus subtilis;

D O I：

10.1007/s004380050745

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA sequences upstream (UP element) of the core promoter (-10, -35 region) of the Bacillus subtilis flagellin gene hag stimulate transcription in vivo and in vitro. We constructed a number of hybrids, placing the UP element of hagp upstream of the core of one SigD-dependent (fliDp) and two SigA-dependent (tmsp, vegp) B. subtilis promoters. The hybrid promoters were fused to a lacZ reporter gene and their activity tested in vivo. The presence of the UP module enhanced transcription at both types of promoters. We conclude that the hagp UP sequence can act as a promoter module independently of the core sequence.

引用

页码：385 / 388

页数：4

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