Temporal control of cell-specific transgene expression in Caenorhabditis elegans

被引：42

作者：

Bacaj, Taulant ^{[1
]}

Shaham, Shai ^{[1
]}

机构：

[1] Rockefeller Univ, Lab Dev Genet, New York, NY 10021 USA

来源：

GENETICS | 2007年 / 176卷 / 04期

关键词：

D O I：

10.1534/genetics.107.074369

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学]; 090102 [作物遗传育种];

摘要：

Cell-specific promoters allow only spatial control of transgene expression in Caenorhabditis elegans. We describe a method, using cell-specific rescue of heat-shock factor-1 (hsf-1) mutants, that allows spatial and temporal regulation of transgene expression. We demonstrate the utility of this method for timed reporter gene expression and for temporal studies of gene function.

引用

页码：2651 / 2655

页数：5

共 26 条

[1]

The molecular identities of the Caenorhabditis elegans intraflagellar transport genes dyf-6, daf-10 and osm-1 [J].

Bell, Leslie R. ;

Stone, Steven ;

Yochem, John ;

Shaw, Jocelyn E. ;

Herman, Robert K. .

GENETICS, 2006, 173 (03) :1275-1286

[2]

BRAND AH, 1993, DEVELOPMENT, V118, P401

[3]

Collet J, 1998, GENETICS, V148, P187

[4]

Ligand-activated site-specific recombination in mice [J].

Feil, R ;

Brocard, J ;

Mascrez, B ;

LeMeur, M ;

Metzger, D ;

Chambon, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (20) :10887-10890

[5]

A MODULAR SET OF LACZ FUSION VECTORS FOR STUDYING GENE-EXPRESSION IN CAENORHABDITIS-ELEGANS [J].

FIRE, A ;

HARRISON, SW ;

DIXON, D .

GENE, 1990, 93 (02) :189-198

[6]

Fujiwara M, 1999, DEVELOPMENT, V126, P4839

[7]

TIGHT CONTROL OF GENE-EXPRESSION IN MAMMALIAN-CELLS BY TETRACYCLINE-RESPONSIVE PROMOTERS [J].

GOSSEN, M ;

BUJARD, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) :5547-5551

[8]

TRANSCRIPTIONAL ACTIVATION BY TETRACYCLINES IN MAMMALIAN-CELLS [J].

GOSSEN, M ;

FREUNDLIEB, S ;

BENDER, G ;

MULLER, G ;

HILLEN, W ;

BUJARD, H .

SCIENCE, 1995, 268 (5218) :1766-1769

[9]

The L-type cyclin CYL-1 and the heat-shock-factor HSF-1 are required for heat-shock-induced protein expression in Caenorhabditis elegans [J].

Hajdu-Cronin, YM ;

Chen, WJ ;

Sternberg, PW .

GENETICS, 2004, 168 (04) :1937-1949

[10]

Targeted gene expression without a tissue-specific promoter: Creating mosaic embryos using laser-induced single-cell heat shock [J].

Halfon, MS ;

Kose, H ;

Chiba, A ;

Keshishian, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (12) :6255-6260

← 1 2 3 →