Highly sensitive PCR assay for routine diagnosis of African swine fever virus in clinical samples

被引：199

作者：

Agüero, M

Fernández, J

Romero, L

Mascaraque, CS

Arias, M

Sánchez-Vizcaíno, JM

机构：

[1] CISA, INIA, Madrid 28130, Spain

[2] Univ Complutense Madrid, Fac Vet, E-28040 Madrid, Spain

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 09期

关键词：

D O I：

10.1128/JCM.41.9.4431-4434.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This work provides a novel, highly sensitive, hot start PCR method for rapid and specific detection of African swine fever virus (ASFV) that can be used as a routine diagnostic test for ASFV in surveillance, control, and eradication programs. A confirmatory test of the specificity of this method based on restriction endonuclease analysis was also developed.

引用

页码：4431 / 4434

页数：4

共 19 条

[1]

Arias M, 2002, TRENDS IN EMERGING VIRAL INFECTIONS OF SWINE, P133

[2]

Arias M, 2002, TRENDS IN EMERGING VIRAL INFECTIONS OF SWINE, P119

[3]

ARIAS M., 2002, CURSO DIGITAL ENFERM

[4]

Arias M., 1986, MED VET, V3, P333