Detection of Tn917-like sequences within a Tn916-like conjugative transposon (Tn3872) in erythromycin-resistant isolates of Streptococcus pneumoniae

被引:78
作者
McDougal, LK
Tenover, FC
Lee, LN
Rasheed, JK
Patterson, JE
Jorgensen, JH
LeBlanc, DJ
机构
[1] Ctr Dis Control & Prevent, Nosocomial Pathogens Lab Branch G08, Hosp Infect Program, Atlanta, GA 30333 USA
[2] Univ Texas, Hlth Sci Ctr, Dept Med, San Antonio, TX 78284 USA
[3] Univ Texas, Hlth Sci Ctr, Dept Pathol, San Antonio, TX 78284 USA
[4] Indiana Univ, Sch Dent, Dept Oral Biol, Indianapolis, IN 46202 USA
关键词
D O I
10.1128/AAC.42.9.2312
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A series of macrolide-lincosamide-streptogramin B (MLS)-resistant pneumococcal isolates of a variety of serotypes was examined and was found to contain Tn917-like elements by DNA-DNA hybridization. Like Tn1545, Tn917 also encodes an ermAM gene but does not mediate resistance to other antimicrobial agents. Furthermore, nucleotide sequence analyses of the DNAs flanking three of the Tn917-like elements revealed that they were inserted into orf9 of a Tn916-like element in a composite transposon-like structure (Tn3872). Other MLS-resistant strains appeared to contain Tn1545-like elements that had suffered a deletion of sequences including the aphA-3 sequences responsible for kanamycin resistance. Thus, the MLS resistance phenotype in pneumococci appears to be mediated by the ermAM present on a much wider variety of genetic elements than was previously appreciated.
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页码:2312 / 2318
页数:7
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