Fluostatins A and B, new inhibitors of dipeptidyl peptidase III, produced by Streptomyces sp. TA-3391 -: I.: Taxonomy of producing strain, production, isolation, physico-chemical properties and biological properties

被引：30

作者：

Akiyama, T ^{[1
]}

Harada, S ^{[1
]}

Kojima, F ^{[1
]}

Takahashi, Y ^{[1
]}

Imada, C ^{[1
]}

Okami, Y ^{[1
]}

Muraoka, Y ^{[1
]}

Aoyagi, T ^{[1
]}

Takeuchi, T ^{[1
]}

机构：

[1] Inst Microbial Chem, Shinagawa Ku, Tokyo 141, Japan

来源：

JOURNAL OF ANTIBIOTICS | 1998年 / 51卷 / 06期

关键词：

D O I：

10.7164/antibiotics.51.553

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

New inhibitors of dipeptidyl peptidaseIII (EC 3.4.14.4) from human placenta, designated as fluostatins A and B, were discovered in the fermentation broth of a strain isolated in our institute. The strain has been identified as Streptomyces sp. TA-3391 on the basis of taxonomic studies. Fluostatins A and B were purified by Diaion HP-20 chromatography, ethyl acetate extraction, silica gel chromatography and reverse phase preparative HPLC. With the synthetic substrate, arginyl-arginine-2-naphthylamide, the IC50 values of fluostatins A and B were 0.44 and 24.0 mu g/ml, respectively. Fluostatins A and B were slightly inhibitory against other dipeptidyl peptidases. Fluostatin A showed mixed-type (competitive and noncompeptitive) inhibition was human leucine-enkephalin as a substrate, and the inhibition constant (Ki) was 14.2 mu M.

引用

页码：553 / 559

页数：7

共 14 条

[1] Fluostatins A and B, new inhibitors of dipeptidyl peptidase III, produced by Streptomyces sp. TA-3391 -: II.: Structure determination [J].