Metabolic alterations in cerebrospinal fluid from double hemorrhage model of dogs

被引:12
作者
Yin, W [1 ]
Tibbs, R [1 ]
Aoki, K [1 ]
Badr, A [1 ]
Zhang, J [1 ]
机构
[1] Univ Mississippi, Med Ctr, Dept Neurosurg, Jackson, MS 39216 USA
关键词
glucose; lactate; pyruvate; glutamate; CSF; vasospasm;
D O I
10.1179/016164101101198181
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Even though cerebral vasospasm after subarachnoid hemorrhage (SAH) causes cerebral ischemia or infarction, the metabolic alterations in cerebrospinal fluids (CSF) after SAH have not been studied. This study was undertaken to measure the levels of glucose, lactate, pyruvate and glutamate in CSF from double hemorrhage dog models. Thirty-two mongrel dogs of either sex, weighing 18-24 kg, underwent double hemorrhage by percutaneous needle puncture of the cisterna magna and injection of autologous blood on day 0 and day 2. The dogs were then sacrificed on day 3, 5 and 7 after collecting CSF. In another study, the dogs were treated with mitogen-activated protein kinase (MAPK) inhibitors PD98059 and U0126, and caspase-2 and caspase-3 inhibitors from day 3 to day 6 after initial blood injection. CSF was collected on day 7 before dogs were sacrificed. The concentration of glucose, lactate, pyruvate and glutamate in CSF was measured by photometrical method. Compared with CSF collected on day 0, glucose was decreased on days 5-7, lactate was increased on days 2-7, pyruvate was increased on days 2-7, and glutamate was increased on days 3-7 (p < 0.05). In the groups treated with MAPK or caspase inhibitors, most of the metabolic alterations remained unchanged as compared with CSF from untreated dogs. Clinically, caspase inhibitors-2 and -3, and MAPK inhibitor U0126 all failed to prevent vasospasm. MAPK inhibitor PD98059 partially prevented vasospasm. Our data demonstrated a metabolic alteration of glucose, glutamate, lactate and pyruvate in CSF during cerebral vasospasm. This metabolic change is consistent with the time course of cerebral vasospasm. This study suggests that brain energy metabolites and excitative amino acids are altered during cerebral vasospasm.
引用
收藏
页码:87 / 92
页数:6
相关论文
共 17 条
[1]  
Alessandri B, 1999, ACT NEUR S, V75, P21
[2]  
Alessandri B, 1999, ACT NEUR S, V75, P25
[3]   Molecular keys to the problems of cerebral vasospasm [J].
Dietrich, HH ;
Dacey, RG Jr .
NEUROSURGERY, 2000, 46 (03) :517-530
[4]   Activation of protein kinases in canine basilar artery in vasospasm [J].
Fujikawa, H ;
Tani, E ;
Yamaura, I ;
Ozaki, I ;
Miyaji, K ;
Sato, M ;
Takahashi, K ;
Imajoh-Ohmi, S .
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1999, 19 (01) :44-52
[5]  
LeBlanc MH, 1997, BIOL NEONATE, V72, P243
[6]   Predictive value of cerebrospinal fluid (CSF) lactate level versus CSF/blood glucose ratio for the diagnosis of bacterial meningitis following neurosurgery [J].
Leib, SL ;
Boscacci, R ;
Gratzl, O ;
Zimmerli, W .
CLINICAL INFECTIOUS DISEASES, 1999, 29 (01) :69-74
[7]  
Matsuishi T, 1996, ACTA NEUROL SCAND, V93, P72
[8]  
Mayberg MR, 1998, NEUROSURG CLIN N AM, V9, P615
[9]   Adrenocorticotropic hormone therapy for infantile spasms alters pyruvate metabolism in the central nervous system [J].
Miyazaki, M ;
Hashimoto, T ;
Yoneda, Y ;
Saijio, T ;
Mori, K ;
Ito, M ;
Kuroda, Y .
BRAIN & DEVELOPMENT, 1998, 20 (05) :312-318
[10]   Oxyhemoglobin-induced apoptosis in cultured endothelial cells [J].
Ogihara, K ;
Zubkov, AY ;
Bernanke, DH ;
Lewis, AI ;
Parent, AD ;
Zhang, JH .
JOURNAL OF NEUROSURGERY, 1999, 91 (03) :459-465