Cell sheet engineering: Intelligent polymer patterned surfaces for tissue engineered liver

被引：10

作者：

Harimoto, M ^{[1
]}

Yamato, M ^{[1
]}

Kikuchi, A ^{[1
]}

Okano, T ^{[1
]}

机构：

[1] Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo 1628666, Japan

来源：

MACROMOLECULAR SYMPOSIA | 2003年 / 195卷

关键词：

cell sheet; co-culture; hepatocyte; poly (N-isopropylacrylamide); temperature-responsive culture surface;

D O I：

10.1002/masy.200390127

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

We describe a new culture system utilizing the temperature-responsive polymer grafted surface for designing of cell position and layered tissue reconstruction. Organizing of the hepatic tissue structure by controlling the culture system, that is patterned co-culture and layered cell sheet co-culture achieved by moving the cultured cells from the culture surface, resulted in regulation of the hepatocyte function. The technique for cell sheet manipulation would promote the liver tissue engineering in quality.

引用

页码：231 / 235

页数：5

共 6 条

[1] TEMPERATURE-DEPENDENCE OF SWELLING OF CROSS-LINKED POLY(N,N'-ALKYL SUBSTITUTED ACRYLAMIDES) IN WATER [J].

BAE, YH ;

OKANO, T ;

KIM, SW .

JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS, 1990, 28 (06) :923-936

[2]

HARIMOTO M, 2002, IN PRESS J BIOMED MA

[3] Creation of designed shape cell sheets that are noninvasively harvested and moved onto another surface [J].

Hirose, M ;

Kwon, OH ;

Yamato, M ;

Kikuchi, A ;

Okano, T .

BIOMACROMOLECULES, 2000, 1 (03) :377-381

[4]

YAMADA N, 1990, MAKROMOL CHEM-RAPID, V11, P571

[5]

Yamato M, 1999, J BIOMED MATER RES, V44, P44, DOI 10.1002/(SICI)1097-4636(199901)44:1<44::AID-JBM5>3.3.CO

[6]

2-O

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