Protein components of a rat brain synaptic junctional protein preparation

被引：56

作者：

Langnaese, K

Seidenbecher, C

Wex, H

Seidel, B

Hartung, K

Appeltauer, U

Garner, A

Voss, B

Mueller, B

Garner, CC

Gundelfinger, ED

机构：

[1] FED INST NEUROBIOL, DEPT NEUROCHEM & MOL BIOL, D-39008 MAGDEBURG, GERMANY

[2] UNIV HAMBURG, CTR MOL NEUROBIOL, D-20246 HAMBURG, GERMANY

[3] UNIV ALABAMA, NEUROBIOL RES CTR, BIRMINGHAM, AL 35213 USA

来源：

MOLECULAR BRAIN RESEARCH | 1996年 / 42卷 / 01期

关键词：

synapse; postsynaptic density; presynaptic grid; cortex; cytoskeleton; synapse-associated protein; cDNA cloning; expression screening; rat brain;

D O I：

10.1016/S0169-328X(96)00147-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Antisera against a rat brain synaptic protein preparation, the postsynaptic density (PSD) fraction, were used to isolate cDNA clones by expression screening of a rat brain cDNA library. About one fifth of more than 200 analyzed cDNAs encoding potential synapse-associated proteins were previously unknown. Identifiable proteins include, among others, components of the pre- and postsynaptic cytoskeleton, synaptic vesicle proteins and several protein kinases and kinase substrates. This demonstrates that both pre- and postsynaptic elements purify with the PSD fraction.

引用

页码：118 / 122

页数：5

共 27 条

[1] BIOCHEMICAL-CHARACTERIZATION AND LOCALIZATION OF A NON-NORMAL-METHYL-D-ASPARTATE GLUTAMATE RECEPTOR IN RAT-BRAIN [J].