Nkx2.2 antisense RNA overexpression enhanced oligodendrocytic differentiation

被引：71

作者：

Tochitani, Shiro ^{[1
,2
,3
]}

Hayashizaki, Yoshihide ^{[1
,2
,3
,4
]}

机构：

[1] RIKEN, Yokohama Inst, Omics Sci Ctr,Tsurumi Ku, LSA Technol Dev Unit, Yokohama, Kanagawa 2300045, Japan

[2] RIKEN, Yokohama Inst,Frontier Res System, Funct RNA Res Program, RNA Resource Explorat Lab, Kanagawa 2300045, Japan

[3] RIKEN, Yokohama Inst,Genom Sci Ctr, Genome Explorat Res Grp, Tsurumi Ku, Kanagawa 2300045, Japan

[4] RIKEN, Wako Inst, Discovery Res Inst, Genome Sci Lab, Wako, Saitama 3510198, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 372卷 / 04期

关键词：

non-coding RNA; antisense; differentiation; neural stem cell; Nkx2.2;

D O I：

10.1016/j.bbrc.2008.05.127

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nkx2.2AS is an endogenous antisense transcript to Nkx2.2 gene, subcellularly located in the cytoplasm. We tested if Nkx2.2AS transcripts have any biological function by regulating transcription level of Nkx2.2 coding gene in the differentiation of neural stem cells. Forced expression of Nkx2.2AS in neural stem cells enhanced induction of differentiation along lineage of oligodendrocytes. Further analyses showed that overexpression of Nkx2.2AS induced modest increase in Nkx2.2 mRNA level, suggesting that the upregulation of Nkx2.2 mRNA level can be a minor cause of enhanced induction of oligodendrocytic differentiation by Nkx2.2AS overexpression. These results together imply that Nkx2.2AS has a certain biological function which is likely to be not only based on the affect on transcription level of Nkx2.2 coding but also on the other mechanisms. gene in cis, but also on the other mechanisms. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：691 / 696

页数：6

共 26 条

[1] A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial-mesenchymal transition [J].

Beltran, Manuel ;

Puig, Isabel ;

Pena, Cristina ;

Miguel Garcia, Jose ;

Belen Alvarez, Ana ;

Pena, Raul ;

Bonilla, Felix ;

Garcia de Herreros, Antonio .

GENES & DEVELOPMENT, 2008, 22 (06) :756-769

[2] THE HUMAN XIST GENE - ANALYSIS OF A 17 KB INACTIVE X-SPECIFIC RNA THAT CONTAINS CONSERVED REPEATS AND IS HIGHLY LOCALIZED WITHIN THE NUCLEUS [J].

BROWN, CJ ;

HENDRICH, BD ;

RUPERT, JL ;

LAFRENIERE, RG ;

XING, Y ;

LAWRENCE, J ;

WILLARD, HF .

CELL, 1992, 71 (03) :527-542

[3]

Cao XW, 2006, ANNU REV NEUROSCI, V29, P77, DOI 10.1146/annurev.neuro.29.051605.112839

[4] The transcriptional landscape of the mammalian genome [J].

Carninci, P ;

Kasukawa, T ;

Katayama, S ;

Gough, J ;

Frith, MC ;

Maeda, N ;

Oyama, R ;

Ravasi, T ;

Lenhard, B ;

Wells, C ;

Kodzius, R ;

Shimokawa, K ;

Bajic, VB ;

Brenner, SE ;

Batalov, S ;

Forrest, ARR ;

Zavolan, M ;

Davis, MJ ;

Wilming, LG ;

Aidinis, V ;

Allen, JE ;

Ambesi-Impiombato, X ;

Apweiler, R ;

Aturaliya, RN ;

Bailey, TL ;

Bansal, M ;

Baxter, L ;

Beisel, KW ;

Bersano, T ;

Bono, H ;

Chalk, AM ;

Chiu, KP ;

Choudhary, V ;

Christoffels, A ;

Clutterbuck, DR ;

Crowe, ML ;

Dalla, E ;

Dalrymple, BP ;

de Bono, B ;

Della Gatta, G ;

di Bernardo, D ;

Down, T ;

Engstrom, P ;

Fagiolini, M ;

Faulkner, G ;

Fletcher, CF ;

Fukushima, T ;

Furuno, M ;

Futaki, S ;

Gariboldi, M .

SCIENCE, 2005, 309 (5740) :1559-1563

[5] Unbiased mapping of transcription factor binding sites along human chromosomes 21 and 22 points to widespread regulation of noncoding RNAs [J].

Cawley, S ;

Bekiranov, S ;

Ng, HH ;

Kapranov, P ;

Sekinger, EA ;

Kampa, D ;

Piccolboni, A ;

Sementchenko, V ;

Cheng, J ;

Williams, AJ ;

Wheeler, R ;

Wong, B ;

Drenkow, J ;

Yamanaka, M ;

Patel, S ;

Brubaker, S ;

Tammana, H ;

Helt, G ;

Struhl, K ;

Gingeras, TR .

CELL, 2004, 116 (04) :499-509

[6]

Fukuda Shinji, 2005, Anatomical Science International, V80, P12

[7] Coupling of cell migration with neurogenesis by proneural bHLH factors [J].

Ge, WH ;

He, F ;

Kim, KJ ;

Blanchi, B ;

Coskun, V ;

Nguyen, L ;

Wu, XB ;

Zhao, J ;

Heng, JIT ;

Martinowich, K ;

Tao, JF ;

Wu, H ;

Castro, D ;

Sobeih, MM ;

Corfas, G ;

Gleeson, JG ;

Greenberg, ME ;

Guillemot, F ;

Sun, YE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (05) :1319-1324

[8] Cell fate specification in the mammalian telencephalon [J].

Guillemot, Francois .

PROGRESS IN NEUROBIOLOGY, 2007, 83 (01) :37-52

[9] The expanding universe of noncoding RNAs [J].

Hannon, G. J. ;

Rivas, F. V. ;

Murchison, E. P. ;

Steitz, J. A. .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 2006, 71 :551-564

[10] Antisense transcription in the mammalian transcriptome [J].

Katayama, S ;

Tomaru, Y ;

Kasukawa, T ;

Waki, K ;

Nakanishi, M ;

Nakamura, M ;

Nishida, H ;

Yap, CC ;

Suzuki, M ;

Kawai, J ;

Suzuki, H ;

Carninci, P ;

Hayashizaki, Y ;

Wells, C ;

Frith, M ;

Ravasi, T ;

Pang, KC ;

Hallinan, J ;

Mattick, J ;

Hume, DA ;

Lipovich, L ;

Batalov, S ;

Engström, PG ;

Mizuno, Y ;

Faghihi, MA ;

Sandelin, A ;

Chalk, AM ;

Mottagui-Tabar, S ;

Liang, Z ;

Lenhard, B ;

Wahlestedt, C .

SCIENCE, 2005, 309 (5740) :1564-1566

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