Interaction of translation initiation factor 3 with the 30S ribosomal subunit

被引：155

作者：

Dallas, A ^{[1
]}

Noller, HF ^{[1
]}

机构：

[1] Univ Calif Santa Cruz, Ctr Mol Biol RNA, Sinsheimer Labs, Santa Cruz, CA 95064 USA

来源：

MOLECULAR CELL | 2001年 / 8卷 / 04期

关键词：

D O I：

10.1016/S1097-2765(01)00356-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydroxyl radical footprinting and directed probing from Fe(II)-derivatized IF3 have been used to map the interaction of IF3 relative to 16S rRNA and tRNA(f)(Met) in the 30S ribosomal subunit. Our results place the two domains of IF3 on opposite sides of the initiator tRNA, with the C domain at the platform interface and the N domain at the E site. The C domain coincides with the location of helix 69 of 23S rRNA, explaining the ability of IF3 to block subunit association. The N domain neighbors proteins S7 and S11 and may interfere with E site tRNA binding. Our model suggests that IF3 influences initiator tRNA selection indirectly.

引用

页码：855 / 864

页数：10

共 49 条

[1] X-RAY CRYSTALLOGRAPHY SHOWS THAT TRANSLATIONAL INITIATION-FACTOR IF3 CONSISTS OF 2 COMPACT ALPHA/BETA DOMAINS LINKED BY AN ALPHA-HELIX [J].

BIOU, V ;

SHU, F ;

RAMAKRISHNAN, V .

EMBO JOURNAL, 1995, 14 (16) :4056-4064

[2] DIRECT CROSS-LINKS BETWEEN INITIATION FACTOR-I, FACTOR-II, AND FACTOR-III AND RIBOSOMAL-PROTEINS PROMOTED BY 2-IMINOTHIOLANE [J].

BOILEAU, G ;

BUTLER, P ;

HERSHEY, JWB ;

TRAUT, RR .

BIOCHEMISTRY, 1983, 22 (13) :3162-3170

[3] ALTERNATIVE OCCUPANCY OF A DUAL RIBOSOMAL-BINDING SITE BY MESSENGER-RNA AFFECTED BY TRANSLATION INITIATION-FACTORS [J].

CANONACO, MA ;

GUALERZI, CO ;

PON, CL .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 182 (03) :501-506

[4] MECHANISM OF TRANSLATIONAL INITIATION IN PROKARYOTES - EVIDENCE FOR A DIRECT EFFECT OF IF2 ON THE ACTIVITY OF THE 30-S RIBOSOMAL-SUBUNIT [J].

CANONACO, MA ;

CALOGERO, RA ;

GUALERZI, CO .

FEBS LETTERS, 1986, 207 (02) :198-204

[5] Crystal structure of an initiation factor bound to the 30S ribosomal subunit [J].

Carter, AP ;

Clemons, WM ;

Brodersen, DE ;

Morgan-Warren, RJ ;

Hartsch, T ;

Wimberly, BT ;

Ramakrishnan, V .

SCIENCE, 2001, 291 (5503) :498-501

[6] Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics [J].

Carter, AP ;

Clemons, WM ;

Brodersen, DE ;

Morgan-Warren, RJ ;

Wimberly, BT ;

Ramakrishnan, V .

NATURE, 2000, 407 (6802) :340-348

[7] X-ray crystal structures of 70S ribosome functional complexes [J].

Cate, JH ;

Yusupov, MM ;

Yusupova, GZ ;

Earnest, TN ;

Noller, HF .

SCIENCE, 1999, 285 (5436) :2095-2104

[8] IF-3 CROSSLINKING TO ESCHERICHIA-COLI RIBOSOMAL 30-S SUBUNITS BY 3 DIFFERENT LIGHT-DEPENDENT PROCEDURES - IDENTIFICATION OF 30-S-PROTEINS CROSSLINKED TO IF-3 - UTILIZATION OF A NEW 2-STAGE CROSSLINKING REAGENT, PARA-NITROBENZYLMALEIMIDE [J].

COOPERMAN, BS ;

EXPERTBEZANCON, A ;

KAHAN, L ;

DONDON, J ;

GRUNBERGMANAGO, M .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1981, 208 (02) :554-562

[9] PHOTOSENSITIZED CROSS-LINKING OF IF-3 TO ESCHERICHIA-COLI 30S SUBUNITS [J].

COOPERMAN, BS ;

DONDON, J ;

FINELLI, J ;

GRUNBERGMANAGO, M ;

MICHELSON, AM .

FEBS LETTERS, 1977, 76 (01) :59-63

[10]

Culver GM, 2000, METHOD ENZYMOL, V318, P461

← 1 2 3 4 5 →