Development of an evanescent-field fibre optic sensor for Escherichia coli O157:H7

被引：35

作者：

Ferreira, AP ^{[1
]}

Werneck, MM

Ribeiro, RM

机构：

[1] Natl Sch Publ Hltl, Oswaldo Cruz Fdn, Dept Sanitat & Environm Hlth, Rio De Janeiro, Brazil

[2] Fed Univ Rio De Janeiro, Biomed Engn Program, BR-21945970 Rio De Janeiro, Brazil

来源：

BIOSENSORS & BIOELECTRONICS | 2001年 / 16卷 / 06期

关键词：

fibre optic sensor; evanescent field; Escherichia coli O157 : H7;

D O I：

10.1016/S0956-5663(01)00149-X

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An intensity-modulated fibre optic sensor was developed for Escherichia coli O157:H7. The interaction between the whole natural bacteria and the guided lightwave was carried out by means of evanescent-field coupling. A correlation between optical response and the current number of bacteria was achieved. The device sensitivity had been calibrated for initial number of bacteria (N-0) from 10-800. The sensor sensitivity was 0.016 (+/- 0.001) dB/h/N-0. The sensing mechanism starts together with the log phase leading the present sensor response to be five to ten times faster than conventional bacteriological techniques. (C) 2001 Elsevier Science BY. All rights reserved.

引用

页码：399 / 408

页数：10

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