Microchip enzymatic assay of organophosphate nerve agents

被引：51

作者：

Wang, J ^{[1
]}

Chen, G

Muck, A

Chatrathi, MP

Mulchandani, A

Chen, W

机构：

[1] New Mexico State Univ, Dept Chem & Biochem, Las Cruces, NM 88003 USA

[2] Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USA

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 505卷 / 02期

基金：

美国国家卫生研究院;

关键词：

organophosphate; nerve agents; enzymatic assay;

D O I：

10.1016/j.aca.2003.10.077

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An on-chip enzymatic assay for screening organophosphate (OP) nerve agents, based on a pre-column reaction of organophosphorus hydrolase (OPH), electrophoretic separation of the phosphonic acid products, and their contactless-conductivity detection, is described. Factors affecting the enzymatic reaction, the separation and detection processes have been assessed and optimized. The complete bioassay requires 1 min of the OPH reaction, along with 1-2 min for the separation and detection of the reaction products. The response is linear, with detection limits of 5 and 3 mg/l for paraoxon and methyl parathion, respectively. Compared to conventional OPH-based biosensors, the OPH-biochip can differentiate between the individual OP substrates. The attractive behavior of the new OPH-based biochip indicates great promise for field screening of OP pesticides and nerve agents. The study demonstrates also for the first time the suitability of the contactless-conductivity detection for on-chip monitoring of enzymatic reactions. (C) 2003 Elsevier B.v. All rights reserved.

引用

页码：183 / 187

页数：5

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