In vitro plant regeneration from cotyledon explants of Swainsona salsula Taubert

被引：25

作者：

Yang, J ^{[1
]}

Hu, Z ^{[1
]}

Guo, GQ ^{[1
]}

Zheng, GC ^{[1
]}

机构：

[1] Lanzhou Univ, Sch Life Sci, Inst Cell Biol, Lanzhou 730000, Peoples R China

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2001年 / 66卷 / 01期

基金：

中国国家自然科学基金;

关键词：

organogenesis; plant regeneration; Swainsona salsula Taubert;

D O I：

10.1023/A:1010679500619

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An effective protocol has been developed for plant regeneration from cotyledon explants of Swainsona salsula Taubert (Saline swainsona), a medicinal and agronomic shrub. Adventitious shoots were obtained from 83.2% of cotyledon explants from 3-day seedlings cultured on Murashige and Skoog (MS) medium containing 2.0 mg l(-1) thidiazuron (TDZ), with an average of 9.3 shoots per explant. Individual elongated shoots were rooted on half strength MS medium supplemented with 2.0 mg l(-1) indole-3-butyric acid (IBA), with 59.3% success. Regenerated plants with well developed shoots and roots were successfully transferred to soil, without detectable variants. Histological observation revealed that shoots developed from cotyledon explants via organogenesis, with little callus.

引用

页码：35 / 39

页数：5

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