Micellar electrokinetic capillary chromatography and data alignment analysis: a new tool in urine profiling

被引：28

作者：

Guillo, C

Barlow, D

Perrett, D

Hanna-Brown, M ^{[1
]}

机构：

[1] Kings Coll London, Dept Pharm, London SE1 9NN, England

[2] Barts & London Queen Marys Sch Med & Dent, Dept Med, London EC1A, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1027卷 / 1-2期

关键词：

electrokinetic chromatography; data alignment; cyclodextrins;

D O I：

10.1016/j.chroma.2003.11.102

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The complex nature of biofluids demands efficient, sensitive and high-resolution analytical methodologies to examine how the 'metabolic fingerprint' changes during disease. This paper describes how sulphated beta-cyclodextrin-modified micellar electrokinetic capillary chromatography (SbetaCD-MECC) has been combined with data alignment analysis and may prove a useful new tool in urine profiling, allowing for separation of over 80 urinary analytes in under 25 min. The optimised and validated SbetaCD-MECC methodology combined with data alignment analysis provides rapid identification of 'mismatches' between urine profiles which are not easily detected with the naked eye as well as a 'similarity score' which indicates the total sum of differences between one profile and another. The combination of SbetaCD-MECC with data alignment software should prove a useful alternative tool in metabonomic studies for rapid comparison of urine profiles. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：203 / 212

页数：10

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