Structure and bioactivity of the polysaccharides in medicinal plant Dendrobium huoshanense

被引：177

作者：

Hsieh, Yves S. -Y. ^{[1
]}

Chien, Cheng ^{[1
,2
]}

Liao, Sylvian K. -S. ^{[1
]}

Liao, Shih-Fen ^{[1
]}

Hung, Wei-Ting ^{[1
]}

Yang, Wen-Bin ^{[1
]}

Lin, Chih-Chien ^{[1
]}

Cheng, Ting-Jen Rachel ^{[1
]}

Chang, Chia-Chuan ^{[1
]}

Fang, Jim-Min ^{[1
,2
]}

Wong, Chi-Huey ^{[1
]}

机构：

[1] Acad Sinica, Gen Res Ctr, Taipei 115, Taiwan

[2] Natl Taiwan Univ, Dept Chem, Taipei 106, Taiwan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2008年 / 16卷 / 11期

关键词：

Dendrobium huoshanense; cell wall; mucilage; polysaccharide; structure; immune response;

D O I：

10.1016/j.bmc.2008.04.042

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Detailed structures of the active polysaccharides extracted from the leaf and stem cell walls and mucilage of Dendrobium huoshanense are determined by using various techniques, including chromatographic, spectroscopic, chemical, and enzymatic methods. The mucilage polysaccharide exhibits specific functions in activating murine splenocytes to produce several cytokines including IFN-gamma, IL-10, IL-6, and IL-1 alpha, as well as hematopoietic growth factors GM-CSF and G-CSF. However, the deacetylated mucilage obtained from an alkaline treatment fails to induce cytokine production. The structure and bioactivity of mucilage components are validated by further fractionation. This is the first study that provides clear evidence for the structure and activity relationship of the polysaccharide in D. huoshanense. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：6054 / 6068

页数：15

共 48 条

[1] NMR-SPECTROSCOPY IN THE STRUCTURAL ELUCIDATION OF OLIGOSACCHARIDES AND GLYCOSIDES [J].