The mRNA level of the potassium-chloride cotransporter KCC2 covaries with seizure susceptibility in inferior colliculus of the post-ischemic audiogenic seizure-prone rat

被引：19

作者：

Reid, KH ^{[1
]}

Li, GY

Payne, RS

Schurr, A

Cooper, NGF

机构：

[1] Univ Louisville, Sch Med, Dept Anat Sci & Neurobiol, Louisville, KY 40292 USA

[2] Univ Louisville, Sch Med, Dept Pharmacol & Toxicol, Louisville, KY 40292 USA

[3] Univ Louisville, Sch Med, Dept Anesthesiol, Louisville, KY 40292 USA

来源：

NEUROSCIENCE LETTERS | 2001年 / 308卷 / 01期

关键词：

potassium-chloride cotransporter KCC2; seizure susceptibility; post-ischemic audiogenic seizure; rat; inferior colliculus;

D O I：

10.1016/S0304-3940(01)01973-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Cardiac arrest and resuscitation were used to induce brain damage and susceptibility to sound-triggered seizures in Sprague-Dawley rats. Glucose preloading was used to vary seizure susceptibility. Because loop diuretics can block these seizures, we investigated changes in KCC2, a potassium-chloride cotransporter, in the inferior colliculus - the origin of the seizures. Using polymerase chain reaction (PCR), we found that collicular KCC2 mRNA levels covaried with seizure susceptibility in these animals. Using quantitative PCR, we found that a fivefold increase in collicular KCC2 mRNA levels was associated with a doubling of seizure incidence. A hypothesis linking KCC2 activity to seizure susceptibility is presented. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：29 / 32

页数：4

共 14 条

[1] NEUROBIOLOGY OF SEIZURE DISPOSITION - THE GENETICALLY EPILEPSY-PRONE RAT .7. NEUROANATOMICAL LOCALIZATION OF STRUCTURES RESPONSIBLE FOR SEIZURES IN THE GEPR - LESION STUDIES [J].

BROWNING, RA .

LIFE SCIENCES, 1986, 39 (10) :857-867

[2] Molecular cloning and functional expression of the K-Cl cotransporter from rabbit, rat, and human - A new member of the cation-chloride cotransporter family [J].

Gillen, CM ;

Brill, S ;

Payne, JA ;

Forbush, B .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (27) :16237-16244

[3] EARLY, BUT NOT LATE, ANTIEPILEPTIC TREATMENT REDUCES RELAPSE OF SOUND-INDUCED SEIZURES IN THE POSTISCHEMIC RAT [J].

IYER, VG ;

REID, KH ;

YOUNG, C ;

MILLER, J ;

SCHURR, A .

BRAIN RESEARCH, 1995, 689 (01) :159-162

[4] DEVELOPMENT OF SUSCEPTIBILITY TO AUDIOGENIC-SEIZURES FOLLOWING CARDIAC-ARREST CEREBRAL-ISCHEMIA IN RATS [J].

KAWAI, K ;

PENIX, LP ;

KAWAHARA, N ;

RUETZLER, CA ;

KLATZO, I .

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1995, 15 (02) :248-258

[5] In vitro electrophysiology of neurons in subnuclei of rat inferior colliculus [J].

Li, Y ;

Evans, MS ;

Faingold, CL .

HEARING RESEARCH, 1998, 121 (1-2) :1-10

[6] Functional characterization of the neuronal-specific K-Cl cotransporter: implications for [K+](0) regulation [J].

Payne, JA .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1997, 273 (05) :C1516-C1525

[7]

PIATAK M, 1993, BIOTECHNIQUES, V14, P70

[8] Audiogenic seizures following global ischemia induced by chest compression in Long-Evans rats [J].

Reid, KH ;

Young, C ;

Schurr, A ;

Tseng, M ;

Payne, RS ;

Keelen, P ;

Miller, J ;

Iyer, V .

EPILEPSY RESEARCH, 1996, 23 (03) :195-209

[9] Agents which block potassium-chloride cotransport prevent sound-triggered seizures in post-ischemic audiogenic seizure-prone rats [J].

Reid, KH ;

Guo, SZ ;

Iyer, VG .

BRAIN RESEARCH, 2000, 864 (01) :134-137

[10] The K+/Cl- co-transporter KCC2 renders GABA hyperpolarizing during neuronal maturation [J].

Rivera, C ;

Voipio, J ;

Payne, JA ;

Ruusuvuori, E ;

Lahtinen, H ;

Lamsa, K ;

Pirvola, U ;

Saarma, M ;

Kaila, K .

NATURE, 1999, 397 (6716) :251-255

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