Mechanism of anti-angiogenic property of gold nanoparticles: role of nanoparticle size and surface charge

被引:208
作者
Arvizo, Rochelle R. [1 ]
Rana, Subinoy [2 ]
Miranda, Oscar R. [2 ]
Bhattacharya, Resham [1 ]
Rotello, Vincent M. [2 ]
Mukherjee, Priyabrata [1 ,3 ,4 ]
机构
[1] Mayo Clin, Dept Biochem & Mol Biol, Coll Med, Rochester, MN 55905 USA
[2] Univ Massachusetts, Dept Chem, Amherst, MA 01003 USA
[3] Mayo Clin, Dept Physiol & Biomed Engn, Coll Med, Rochester, MN 55905 USA
[4] Mayo Clin, Mayo Clin Canc Ctr, Coll Med, Rochester, MN 55905 USA
关键词
Gold nanoparticles; Angiogenesis; VEGF165; Protein conformation; GROWTH-FACTOR; CANCER; PROTEINS; BIOLOGY; DELIVERY;
D O I
10.1016/j.nano.2011.01.011
中图分类号
TB3 [工程材料学];
学科分类号
082905 [生物质能源与材料];
摘要
Discovering therapeutic inorganic nanoparticles (NPs) is evolving as an important area of research in the emerging field of nanomedicine. Recently, we reported the anti-angiogenic property of gold nanoparticles (GNPs): It inhibits the function of pro-angiogenic heparin-binding growth factors (HB-GFs), such as vascular endothelial growth factor 165 (VEGF165) and basic fibroblast growth factor (bFGF), etc. However, the mechanism through which GNPs imparts such an effect remains to be investigated. Using GNPs of different sizes and surface charges, we demonstrate here that a naked GNP surface is required and core size plays an important role to inhibit the function of HB-GFs and subsequent intracellular signaling events. We also demonstrate that the inhibitory effect of GNPs is due to the change in HB-GFs conformation/configuration (denaturation) by the NPs, whereas the conformations of non-HB-GFs remain unaffected. We believe that this significant study will help structure-based design of therapeutic NPs to inhibit the functions of disease-causing proteins. From the Clinical Editor: In this landmark paper by Arvizo and colleagues, the angiogenesis inhibitor effects of gold nanoparticles were investigated as the function of size and charge. This study will pave the way to the development of therapeutic NPs that inhibit the functions of pathogenic proteins. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:580 / 587
页数:8
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