Isolation and genetic characterization of two G3P5A[3] canine rotavirus strains in Italy

被引：21

作者：

Martella, V ^{[1
]}

Pratelli, A ^{[1
]}

Elia, G ^{[1
]}

Decaro, N ^{[1
]}

Tempesta, M ^{[1
]}

Buonavoglia, C ^{[1
]}

机构：

[1] Univ Bari, Fac Vet Med, Dept Anim Hlth & Wellbeing, Bari, Italy

来源：

JOURNAL OF VIROLOGICAL METHODS | 2001年 / 96卷 / 01期

关键词：

canine rotavirus; VP4; VP7; polymerase chain reaction;

D O I：

10.1016/S0166-0934(01)00312-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two strains of canine rotavirus were isolated from pups with clinical signs of gastroenteritis. Both strains were identified by polymerase chain reaction (PCR) as G3P5A[3], although restriction endonuclease analysis of the PCR amplicons revealed a genetic difference between the two isolates in the VP7 gene. The: isolation in Italy of canine rotaviruses displaying the same VP7 and VP4 specificities as in the USA and in Japan. suggests that the G3 and P5A[3] types are highly conserved among canine rotavirus strains. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：43 / 49

页数：7

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