Engineered human IgG antibodies with longer serum half-lives in primates

被引：216

作者：

Hinton, PR ^{[1
]}

Johlfs, MG ^{[1
]}

Xiong, JM ^{[1
]}

Hanestad, K ^{[1
]}

Ong, KC ^{[1
]}

Bullock, C ^{[1
]}

Keller, S ^{[1
]}

Tang, MT ^{[1
]}

Tso, JY ^{[1
]}

V squez, M ^{[1
]}

Tsurushita, N ^{[1
]}

机构：

[1] Prot Design Labs Inc, Fremont, CA 94555 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2004年 / 279卷 / 08期

关键词：

D O I：

10.1074/jbc.C300470200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The neonatal Fe receptor (FcRn) plays an important role in regulating the serum half-lives of IgG antibodies. A correlation has been established between the pH-dependent binding affinity of IgG antibodies to FcRn and their serum half-lives in mice. In this study, molecular modeling was used to identify Fe positions near the FcRn binding site in a human IgG antibody that, when mutated, might alter the binding affinity of IgG to FcRn. Following mutagenesis, several IgG, mutants with increased binding affinity to human FcRn at pH 6.0 were identified at Fc positions 250 and 428. These mutants do not bind to human FcRn at pH 7.5. A pharmacokinetics study of two mutant IgG(2) antibodies with increased FcRn binding affinity indicated that they had serum half-lives in rhesus monkeys similar to2-fold longer than the wild-type antibody.

引用

页码：6213 / 6216

页数：4

共 34 条

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[Anonymous], [No title captured]

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