Effect of Triptolide on T-Cell Receptor Beta Variable Gene mRNA Expression in Rats With Collagen-Induced Arthritis

被引：27

作者：

Wang, Jixi ^{[1
,2
]}

Wang, Aibing ^{[3
]}

Zeng, Heqing ^{[4
]}

Liu, Lantao ^{[1
]}

Jiang, Wenhao ^{[1
]}

Zhu, Yongjie ^{[5
]}

Xu, Yudong ^{[1
]}

机构：

[1] Harbin Med Univ, Dept Human Anat, Basic Med Coll, Harbin 150086, Heilongjiang Pr, Peoples R China

[2] Heilongjiang Univ Chinese Med, Basic Med Coll, Dept Human Anat, Harbin, Heilongjiang Pr, Peoples R China

[3] Qinghai Prov Peoples Hosp, Dept Nephrol, Xining, Qinghai Provinc, Peoples R China

[4] Hunan Wangwang Hosp, Dept Anesthesia, Changsha, Hunan, Peoples R China

[5] Aksu Prefecture First Peoples Hosp, Dept Pathol, Aksu, Xinjiang Provin, Peoples R China

来源：

ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY | 2012年 / 295卷 / 06期

关键词：

triptolide; collagen-induced arthritis; T-cell receptor; interleukin-10; TRIPTERYGIUM-WILFORDII; RHEUMATOID-ARTHRITIS; INHIBITION; REPERTOIRE; EXPANSION; EXTRACT;

D O I：

10.1002/ar.22479

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100123 [人体微生态学]; 100210 [外科学];

摘要：

Triptolide (TP) has been used in the treatment of rheumatoid arthritis (RA), but its mechanism of action is not understood. T-cell activation and associated release of cytokines appear to be major factors in the pathogenesis of RA. The overexpression of T-cell receptor (TCR) variable gene (V gene) fragments can cause the activation and infiltration of autoreactive T cells. This study examines the effects of TP on rats with collagen-induced arthritis (CIA). The levels of interleukin-10 (IL-10) in the serum were examined with ELISA. Compared to the CIA group, the levels of IL-10 were greater in the TP treatment group. Real-time quantitative polymerase chain reaction confirmed that the expression of TCR V beta (BV) 15 and TCR BV19 was increased in the CIA group, whereas in the TP treatment group, the expression was decreased. In this study, TP was found to enhance IL-10 levels and decrease the expression levels of TCR BV15 and TCR BV19. These changes might help explain the effectiveness of TP in the treatment of RA. Anat Rec, 2012. (c) 2012 Wiley Periodicals, Inc.

引用

页码：922 / 927

页数：6

共 31 条

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