Temperature regulation of virulence factors in the pathogen Vibrio coralliilyticus

被引:202
作者
Kimes, Nikole E.
Grim, Christopher J. [2 ,3 ,4 ]
Johnson, Wesley R. [5 ]
Hasan, Nur A. [2 ]
Tall, Ben D. [4 ]
Kothary, Mahendra H. [4 ]
Kiss, Hajnalka [6 ]
Munk, A. Christine [6 ]
Tapia, Roxanne [6 ]
Green, Lance [6 ]
Detter, Chris [6 ]
Bruce, David C. [6 ]
Brettin, Thomas S. [6 ]
Colwell, Rita R. [2 ,3 ,7 ]
Morris, Pamela J. [1 ]
机构
[1] Univ S Carolina, Belle W Baruch Inst Marine & Coastal Sci, Baruch Marine Field Lab, Georgetown, SC 29442 USA
[2] Univ Maryland, Maryland Pathogen Res Inst, College Pk, MD 20742 USA
[3] Univ Maryland, Inst Adv Comp Studies, Ctr Bioinformat & Computat Biol, College Pk, MD 20742 USA
[4] US FDA, Laurel, MD USA
[5] Ecosyst Solut Inc, Edgewater, MD USA
[6] Los Alamos Natl Lab, Biosci Div, Los Alamos, NM USA
[7] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Baltimore, MD USA
关键词
Vibrio pathogens; coral disease; genome and proteome; quorum sensing; global climate change; temperature; CORAL POCILLOPORA-DAMICORNIS; ACYL HOMOSERINE LACTONE; QUORUM SENSING SIGNAL; CLIMATE-CHANGE; RTX TOXIN; ANTIMICROBIAL RESISTANCE; GENOME SEQUENCE; H-NS; VULNIFICUS; MOTILITY;
D O I
10.1038/ismej.2011.154
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Sea surface temperatures (SST) are rising because of global climate change. As a result, pathogenic Vibrio species that infect humans and marine organisms during warmer summer months are of growing concern. Coral reefs, in particular, are already experiencing unprecedented degradation worldwide due in part to infectious disease outbreaks and bleaching episodes that are exacerbated by increasing SST. For example, Vibrio coralliilyticus, a globally distributed bacterium associated with multiple coral diseases, infects corals at temperatures above 27 degrees C. The mechanisms underlying this temperature-dependent pathogenicity, however, are unknown. In this study, we identify potential virulence mechanisms using whole genome sequencing of V. coralliilyticus ATCC (American Type Culture Collection) BAA-450. Furthermore, we demonstrate direct temperature regulation of numerous virulence factors using proteomic analysis and bioassays. Virulence factors involved in motility, host degradation, secretion, antimicrobial resistance and transcriptional regulation are upregulated at the higher virulent temperature of 27 degrees C, concurrent with phenotypic changes in motility, antibiotic resistance, hemolysis, cytotoxicity and bioluminescence. These results provide evidence that temperature regulates multiple virulence mechanisms in V. coralliilyticus, independent of abundance. The ecological and biological significance of this temperature-dependent virulence response is reinforced by climate change models that predict tropical SST to consistently exceed 27 degrees C during the spring, summer and fall seasons. We propose V. coralliilyticus as a model Gram-negative bacterium to study temperature-dependent pathogenicity in Vibrio-related diseases. The ISME Journal (2012) 6, 835-846; doi: 10.1038/ismej.2011.154; published online 8 December 2011
引用
收藏
页码:835 / 846
页数:12
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