Analysis of Kinetic Behavior of Protein Crystallization in Nanodroplets

被引：12

作者：

Maeki, Masatoshi ^{[1
]}

Yamaguchi, Hiroshi ^{[2
]}

Yamashita, Kenichi ^{[2
]}

Nakamura, Hiroyuki ^{[2
]}

Miyazaki, Masaya ^{[1
,2
]}

Maeda, Hideaki ^{[1
,2
,3
]}

机构：

[1] Kyushu Univ, Dept Mol & Mat Sci, Interdisciplinary Grad Sch Engn Sci, Fukuoka 8168580, Japan

[2] Natl Inst Adv Ind Sci & Technol, Measurement Solut Res Ctr, Saga 8410052, Japan

[3] Japan Sci & Technol Agcy JST, CREST, Chiyoda Ku, Tokyo 1020075, Japan

来源：

CHEMISTRY LETTERS | 2011年 / 40卷 / 08期

基金：

日本科学技术振兴机构;

关键词：

GROWTH; PHASE; POLYDIMETHYLSILOXANE; OPTIMIZATION; THAUMATIN; SILICON;

D O I：

10.1246/cl.2011.825

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This paper demonstrates the possibility of controlling nucleation and crystal growth behavior within a droplet formed in a microspace. The effects of droplet volume and shape were examined by using thaumatin as a model system. The droplet size and shape affected the number and size of the obtained protein crystals.

引用

收藏

页码：825 / 827

页数：3

相关论文

共 15 条

[1] Solubility of thaumatin [J].

Asherie, Neer ;

Ginsberg, Charles ;

Blass, Samuel ;

Greenbaum, Arieh ;

Knafo, Sarah .

CRYSTAL GROWTH & DESIGN, 2008, 8 (06) :1815-1817

[2] Kinetics of protein crystal nucleation and growth in the batch method [J].

Baird, JK ;

Hill, SC ;

Clunie, JC .

JOURNAL OF CRYSTAL GROWTH, 1999, 196 (2-4) :220-225

[3] Surface modification of silicon and polydimethylsiloxane surfaces with vapor-phase-deposited ultrathin fluorosilane films for biomedical nanodevices [J].

Bhushan, Bharat ;

Hansford, Derek ;

Lee, Kang Kug .

JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A, 2006, 24 (04) :1197-1202

[4] High-throughput X-ray crystallography for drug discovery [J].

Blundell, TL ;

Patel, S .

CURRENT OPINION IN PHARMACOLOGY, 2004, 4 (05) :490-496

[5] A practical method for rapid microchannel fabrication in polydimethylsiloxane by replica molding without using silicon photoresist [J].

Briones, Maria Portia P. ;

Honda, Takeshi ;

Yamaguchi, Yoshiko ;

Miyazaki, Masaya ;

Nakamura, Hiroyuki ;

Maeda, Hideaki .

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 2006, 39 (10) :1108-1114

[6] Time evolution of crystallization phase of lysozyme protein in aqueous salt solution as studied by scattering techniques [J].

Chodankar, S. ;

Aswal, V. K. ;

Kohlbrecher, J. ;

Hassan, P. A. ;

Wagh, A. G. .

PHYSICA B-CONDENSED MATTER, 2007, 398 (01) :164-171

[7] Containerless protein crystal growth in rotating levitated drops [J].

Chung, SK ;

Trinh, EH .

JOURNAL OF CRYSTAL GROWTH, 1998, 194 (3-4) :384-397

[8] Rapid prototyping of microfluidic systems in poly(dimethylsiloxane) [J].

Duffy, DC ;

McDonald, JC ;

Schueller, OJA ;

Whitesides, GM .

ANALYTICAL CHEMISTRY, 1998, 70 (23) :4974-4984

[9] A microfluidic device for kinetic optimization of protein crystallization and in situ structure determination [J].

Hansen, CL ;

Classen, S ;

Berger, JM ;

Quake, SR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (10) :3142-3143

[10] High-throughput operation of sample-exchange robots with double tongs at the Photon Factory beamlines [J].

Hiraki, Masahiko ;

Watanabe, Shokei ;

Phonda, Nobuo ;

Yamada, Yusuke ;

Matsugaki, Naohiro ;

Igarashi, Noriyuki ;

Gaponov, Yurii ;

Wakatsuki, Soichi .

JOURNAL OF SYNCHROTRON RADIATION, 2008, 15 :300-303