A method for purification of listeriolysin O from a hypersecretor strain of Listeria monocytogenes

被引：12

作者：

Walton, CM ^{[1
]}

Wu, CH ^{[1
]}

Wu, GY ^{[1
]}

机构：

[1] Univ Connecticut, Ctr Hlth, Dept Med, Div Gastroenterol Hepatol, Farmington, CT 06030 USA

来源：

PROTEIN EXPRESSION AND PURIFICATION | 1999年 / 15卷 / 02期

关键词：

D O I：

10.1006/prep.1998.1022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and convenient method for the purification of the hemolytic toxin listeriolysin O (LLO) from Listeria monocytogenes is described. Supernatants from bacteria cultures were purified by application to a CH2 spiral cartridge concentrator (Amicon) and ion exchange chromatography. A critical step is removal of contaminating RNA. The purified proteins had characteristics described for bacterial thiol-activated hemolysins: activation by a reducing agent (DTT) and inactivation by cholesterol. In addition, the molecular weight of 58,000 and pH-dependent hemolytic activity of this purified protein are consistent with the previously published characteristics of LLO. (C) 1999 Academic Press.

引用

页码：243 / 245

页数：3

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