Changes in the subcellular localization of the Brn4 gene product precede mesenchymal remodeling of the otic capsule

被引:49
作者
Phippard, D
Heydemann, A
Lechner, M
Lu, LH
Lee, D
Kyin, T
Crenshaw, EB
机构
[1] Univ Penn, Dept Neurosci, Philadelphia, PA 19104 USA
[2] Univ Chicago, Dept Mol Genet & Cell Biol, Chicago, IL 60637 USA
关键词
brain-4 (Brn4/Pou3f4); otic capsule; temporal bone formation; immunohistochemistry; in situ hybridization;
D O I
10.1016/S0378-5955(98)00059-8
中图分类号
R36 [病理学]; R76 [耳鼻咽喉科学];
学科分类号
100104 ; 100213 ;
摘要
To better understand the genetic mechanisms that regulate the formation of the temporal bone, we have characterized the developmental expression pattern of the mouse gene, Brn4/Pou3f4, which plays a central role in bony labyrinth formation. Expression of this gene is initially detected in the ventral aspect of the otic capsule at 10.5 days post coitus (dpc), and correlates with the onset of mesenchymal condensation in the oric capsule. As the otic capsule condenses further and surrounds the entire otic vesicle, the Brn4 gene product is detected throughout the inner ear in the mesenchyme of both the cochlear and vestibular aspects. Early in otic embryogenesis, the Brn4 gene product is localized to the nucleus of the vast majority of cells in which it is expressed. The Brn4 gene product remains nuclear in those regions of the otic capsule that eventually give rise to the mature bony labyrinth. However, the subcellular localization of the Brn4 gene product shifts from strictly nuclear to perinuclear in those regions of the otic capsule that will cavitate to form acellular regions in the temporal bone, such as the scala tympani, scala vestibuli, and the internal auditory meatus. These data provide a detailed analysis of the expression pattern of the Brn4 gene, and provide insight into the role of the Brn4 gene product and its regulation during otic capsule formation. (C) 1998 Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:77 / 85
页数:9
相关论文
共 25 条
[1]  
Ausubel F.M., 1991, CURRENT PROTOCOLS MO
[2]   I-KAPPA-B - A SPECIFIC INHIBITOR OF THE NF-KAPPA-B TRANSCRIPTION FACTOR [J].
BAEUERLE, PA ;
BALTIMORE, D .
SCIENCE, 1988, 242 (4878) :540-546
[3]   ASSOCIATION BETWEEN X-LINKED MIXED DEAFNESS AND MUTATIONS IN THE POU DOMAIN GENE POU3F4 [J].
DEKOK, YJM ;
VANDERMAAREL, SM ;
BITNERGLINDZICZ, M ;
HUBER, I ;
MONACO, AP ;
MALCOLM, S ;
PEMBREY, ME ;
ROPERS, HH ;
CREMERS, FPM .
SCIENCE, 1995, 267 (5198) :685-688
[4]   INITIATION OF CHONDROGENESIS IN CULTURED PERIOTIC MESENCHYME - SYNERGISTIC ACTION OF TRANSFORMING GROWTH-FACTOR-BETA AND FIBROBLAST GROWTH-FACTOR [J].
FRENZ, DA ;
WILLIAMS, JD ;
VANDEWATER, TR .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES-SERIES, 1991, 630 :256-258
[5]   TRANSFORMING GROWTH-FACTOR-BETA - PARACRINE MEDIATOR OF OTIC CAPSULE FORMATION [J].
FRENZ, DA ;
GALINOVICSCHWARTZ, V ;
STAECKER, H ;
VANDEWATER, TR .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES-SERIES, 1991, 630 :253-255
[6]   TRANSFORMING GROWTH-FACTOR-BETA - DOES IT DIRECT OTIC CAPSULE FORMATION [J].
FRENZ, DA ;
VANDEWATER, TR ;
GALINOVICSCHWARTZ, V .
ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY, 1991, 100 (04) :301-307
[7]  
FRENZ DA, 1994, DEVELOPMENT, V120, P415
[8]   TRANSFORMING GROWTH-FACTOR BETA-1 IS AN EPITHELIAL-DERIVED SIGNAL PEPTIDE THAT INFLUENCES OTIC CAPSULE FORMATION [J].
FRENZ, DA ;
GALINOVICSCHWARTZ, V ;
LIU, W ;
FLANDERS, KC ;
VANDEWATER, TR .
DEVELOPMENTAL BIOLOGY, 1992, 153 (02) :324-336
[9]   EPITHELIAL CONTROL OF PERIOTIC MESENCHYME CHONDROGENESIS [J].
FRENZ, DA ;
VANDEWATER, TR .
DEVELOPMENTAL BIOLOGY, 1991, 144 (01) :38-46
[10]   EUKARYOTIC PROTEINS EXPRESSED IN ESCHERICHIA-COLI - AN IMPROVED THROMBIN CLEAVAGE AND PURIFICATION PROCEDURE OF FUSION PROTEINS WITH GLUTATHIONE-S-TRANSFERASE [J].
GUAN, KL ;
DIXON, JE .
ANALYTICAL BIOCHEMISTRY, 1991, 192 (02) :262-267