Herpes simplex virus glycoproteins gD and gE/gI serve essential but redundant functions during acquisition of the virion envelope in the cytoplasm

被引:97
作者
Farnsworth, A [1 ]
Goldsmith, K [1 ]
Johnson, DC [1 ]
机构
[1] Oregon Hlth & Sci Univ, Dept Mol Microbiol & Immunol, Portland, OR 97239 USA
关键词
D O I
10.1128/JVI.77.15.8481-8494.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The late stages of assembly of herpes simplex virus (HSV) and other herpesviruses are not well understood. Acquisition of the final virion envelope apparently involves interactions between viral nucleocapsids coated with tegument proteins and the cytoplasmic domains of membrane glycoproteins. This promotes budding of virus particles into cytoplasmic vesicles derived from the trans-Golgi network or endosomes. The identities of viral membrane glycoproteins and tegument proteins involved in these processes are not well known. Here, we report that HSV mutants lacking two viral glycoproteins, gD and gE, accumulated large numbers of unenveloped nucleocapsids in the cytoplasm. These aggregated capsids were immersed in an electron-dense layer that appeared to be tegument. Few or no enveloped virions were observed. More subtle defects were observed with an HSV unable to express gD and gI. A triple mutant lacking gD, gE, and gI exhibited more severe defects in envelopment. We concluded that HSV gD and the gE/gI heterodimeric complex act in a redundant fashion to anchor the virion envelope onto tegument-coated capsids. In the absence of either one of these HSV glycoproteins, envelopment proceeds; however, without both gD and gE, or gE/gI, there is profound inhibition of cytoplasmic envelopment.
引用
收藏
页码:8481 / 8494
页数:14
相关论文
共 54 条
[1]   Deletion of glycoprotein gE reduces the propagation of pseudorabies virus in the nervous system of mice after intranasal inoculation [J].
Babic, N ;
Klupp, B ;
Brack, A ;
Mettenleiter, TC ;
Ugolini, G ;
Flamand, A .
VIROLOGY, 1996, 219 (01) :279-284
[2]   THE OPEN READING FRAMES UL3, UL4, UL10, AND UL16 ARE DISPENSABLE FOR THE REPLICATION OF HERPES-SIMPLEX VIRUS 1 IN CELL-CULTURE [J].
BAINES, JD ;
ROIZMAN, B .
JOURNAL OF VIROLOGY, 1991, 65 (02) :938-944
[3]   AN ANALYSIS OF THE IN-VITRO AND IN WHO PHENOTYPES OF MUTANTS OF HERPES-SIMPLEX VIRUS TYPE-1 LACKING GLYCOPROTEINS GG, GE, GI OR THE PUTATIVE GJ [J].
BALAN, P ;
DAVISPOYNTER, N ;
BELL, S ;
ATKINSON, H ;
BROWNE, H ;
MINSON, T .
JOURNAL OF GENERAL VIROLOGY, 1994, 75 :1245-1258
[4]  
BALL JM, 1995, IN VITRO CELL DEV-AN, V31, P196
[5]   NORMAL KERATINIZATION IN A SPONTANEOUSLY IMMORTALIZED ANEUPLOID HUMAN KERATINOCYTE CELL-LINE [J].
BOUKAMP, P ;
PETRUSSEVSKA, RT ;
BREITKREUTZ, D ;
HORNUNG, J ;
MARKHAM, A ;
FUSENIG, NE .
JOURNAL OF CELL BIOLOGY, 1988, 106 (03) :761-771
[6]   Role of the cytoplasmic tail of pseudorabies virus glycoprotein E in virion formation [J].
Brack, AR ;
Klupp, BG ;
Granzow, H ;
Tirabassi, R ;
Enquist, LW ;
Mettenleiter, TC .
JOURNAL OF VIROLOGY, 2000, 74 (09) :4004-4016
[7]   Inhibition of virion maturation by simultaneous deletion of glycoproteins E, I, and M of pseudorabies virus [J].
Brack, AR ;
Dijkstra, JM ;
Granzow, H ;
Klupp, BG ;
Mettenleiter, TC .
JOURNAL OF VIROLOGY, 1999, 73 (07) :5364-5372
[8]   ROLE OF GLYCOPROTEIN-B OF HERPES-SIMPLEX VIRUS TYPE-1 IN VIRAL ENTRY AND CELL-FUSION [J].
CAL, WH ;
GU, BH ;
PERSON, S .
JOURNAL OF VIROLOGY, 1988, 62 (08) :2596-2604
[9]   ORIGIN OF UNENVELOPED CAPSIDS IN THE CYTOPLASM OF CELLS INFECTED WITH HERPES-SIMPLEX VIRUS-1 [J].
CAMPADELLIFIUME, G ;
FARABEGOLI, F ;
DIGAETA, S ;
ROIZMAN, B .
JOURNAL OF VIROLOGY, 1991, 65 (03) :1589-1595
[10]   PSEUDORABIES VIRUS ENVELOPE GLYCOPROTEIN-GI INFLUENCES BOTH NEUROTROPISM AND VIRULENCE DURING INFECTION OF THE RAT VISUAL-SYSTEM [J].
CARD, JP ;
WHEALY, ME ;
ROBBINS, AK ;
ENQUIST, LW .
JOURNAL OF VIROLOGY, 1992, 66 (05) :3032-3041