Translation of tobacco chloroplast rps14 mRNA depends on a Shine-Dalgarno-like sequence in the 5′-untranslated region but not on internal RNA editing in the coding region

被引：46

作者：

Hirose, T ^{[1
]}

Kusumegi, T ^{[1
]}

Sugiura, M ^{[1
]}

机构：

[1] Nagoya Univ, Ctr Gene Res, Nagoya, Aichi 4648602, Japan

来源：

FEBS LETTERS | 1998年 / 430卷 / 03期

关键词：

chloroplast; Shine-Dalgarno sequence; RNA editing; tobacco; translation;

D O I：

10.1016/S0014-5793(98)00673-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of Shine-Dalgarno-like sequences in mRNAs from higher plant chloroplasts has not been analyzed experimentally so far, In vitro translation analysis has revealed that the Shine-Dalgarno-like sequence is essential for translation of tobacco chloroplast rps14 mRNA. Two RNA editing sites have been identified in the protein-coding region of the rps14 mRNA, Editing of the second site was found to be partial and hence the partially edited transcripts are accumulated in tobacco green leaves. In vitro translation assays using the fully edited, partially edited and unedited rps14 mRNAs indicated that editing does not directly influence translational efficiency. (C) 1998 Federation of European Biochemical Societies.

引用

页码：257 / 260

页数：4

共 31 条

[1] RNA EDITING OF WHEAT MITOCHONDRIAL ATP SYNTHASE SUBUNIT-9 - DIRECT PROTEIN AND CDNA SEQUENCING [J].

BEGU, D ;

GRAVES, PV ;

DOMEC, C ;

ARSELIN, G ;

LITVAK, S ;

ARAYA, A .

PLANT CELL, 1990, 2 (12) :1283-1290

[2] TISSUE-SPECIFIC AND STAGE-SPECIFIC MODULATION OF RNA EDITING OF THE PSBF AND PSBL TRANSCRIPT FROM SPINACH PLASTIDS - A NEW REGULATORY MECHANISM [J].

BOCK, R ;

HAGEMANN, R ;

KOSSEL, H ;

KUDLA, J .

MOLECULAR & GENERAL GENETICS, 1993, 240 (02) :238-244

[3] INTRODUCTION OF A HETEROLOGOUS EDITING SITE INTO THE TOBACCO PLASTID GENOME - THE LACK OF RNA EDITING LEADS TO A MUTANT PHENOTYPE [J].

BOCK, R ;

KOSSEL, H ;

MALIGA, P .

EMBO JOURNAL, 1994, 13 (19) :4623-4628

[4]

Bock R, 1997, EUKARYOTISM SYMBIOSI, P123, DOI [10.1007/978-3-642-60885-8_9, DOI 10.1007/978-3-642-60885-8_9]

[5] TRANSLATION OF CHLOROPLAST-ENCODED MESSENGER-RNA - POTENTIAL INITIATION AND TERMINATION SIGNALS [J].

BONHAMSMITH, PC ;

BOURQUE, DP .

NUCLEIC ACIDS RESEARCH, 1989, 17 (05) :2057-2080

[6] RNA EDITING IN PLANT-MITOCHONDRIA [J].

BONNARD, G ;

GUALBERTO, JM ;

LAMATTINA, L ;

GRIENENBERGER, JM .

CRITICAL REVIEWS IN PLANT SCIENCES, 1992, 10 (06) :503-524

[7] DIFFERENCES IN EDITING AT HOMOLOGOUS SITES IN MESSENGER-RNAS FROM ANGIOSPERM MITOCHONDRIA [J].

COVELLO, PS ;

GRAY, MW .

NUCLEIC ACIDS RESEARCH, 1990, 18 (17) :5189-5196

[8]

Fargo DC, 1998, MOL GEN GENET, V257, P271

[9] RNA EDITING IN MAIZE CHLOROPLASTS IS A PROCESSING STEP INDEPENDENT OF SPLICING AND CLEAVAGE TO MONOCISTRONIC MESSENGER-RNAS [J].

FREYER, R ;

HOCH, B ;

NECKERMANN, K ;

MAIER, RM ;

KOSSEL, H .

PLANT JOURNAL, 1993, 4 (04) :621-629

[10] Occurrence of plastid RNA editing in all major lineages of land plants [J].

Freyer, R ;

KieferMeyer, MC ;

Kossel, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (12) :6285-6290

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