The effect of mechanical strain on protease production by keratinocytes

被引：13

作者：

Bhadal, N. ^{[1
]}

Wall, I. B. ^{[1
]}

Porter, S. R. ^{[1
]}

Broad, S. ^{[2
]}

Lindahl, G. E. ^{[3
]}

Whawell, S. ^{[4
]}

Lewis, M. P. ^{[1
]}

机构：

[1] UCL Eastman Dent Inst, London WC1X 8LD, England

[2] Keratinocyte Res Lab, Canc Res UK, London WC2A 3PX, England

[3] Royal Free & Univ Coll Med Sch, Ctr Rheumatol, London NW3 2PF, England

[4] Univ Sheffield, Sch Clin Dent, Dept Oral Pathol, Sheffield S10 2TA, S Yorkshire, England

来源：

BRITISH JOURNAL OF DERMATOLOGY | 2008年 / 158卷 / 02期

关键词：

keratinocytes; mechanical strain;

D O I：

10.1111/j.1365-2133.2007.08341.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Intact skin is under constant tension, transmitted from the underlying dermis, but when tension is lost (i.e. upon wounding) protease activity is upregulated. To investigate the effect of mechanical strain on protease production by both normal and transformed keratinocytes in vitro. Keratinocytes were seeded on to membranes precoated with either type I or type IV collagen. After 48 h medium was replaced with serum-free medium and mechanical strain was applied. Mechanical strain resulted in decreased urokinase-type plasminogen activator (uPA) production by normal human keratinocytes (P < 0.05) but increased production by transformed keratinocytes (P < 0.05) cultured on type I and type IV collagen. Differential production of uPA by normal and transformed keratinocytes is relevant in the context of normal function, wound healing and tumorigenesis.

引用

页码：396 / 398

页数：3

共 13 条

[1] Matrilysin-2 (matrix metalloproteinase-26) is upregulated in keratinocytes during wound repair and early skin carcinogenesis [J].