Epidermal growth factor receptor controls flat dysplastic aberrant crypt foci development and colon cancer progression in the rat azoxymethane model

被引：44

作者：

Dougherty, Urszula ^{[1
]}

Sehdev, Arnikar ^{[1
]}

Cerda, Sonia ^{[1
]}

Mustafi, Reba ^{[1
]}

Little, Nathaniel ^{[1
]}

Yuan, Weihua ^{[1
]}

Jagadeeswaran, Sujatha ^{[1
]}

Churnsangsri, Anusara ^{[1
]}

Delgado, Jorge ^{[1
]}

Tretiakova, Maria ^{[3
]}

Joseph, Loren ^{[3
]}

Hart, John ^{[3
]}

Cohen, Ezra E. W. ^{[1
,3
]}

Aluri, Lata ^{[1
]}

Fichera, Alessandro ^{[2
]}

Bissonnette, Marc ^{[1
]}

机构：

[1] Univ Chicago, Dept Med, Chicago, IL 60637 USA

[2] Univ Chicago, Dept Surg, Chicago, IL 60637 USA

[3] Univ Chicago, Dept Pathol, Chicago, IL 60637 USA

来源：

CLINICAL CANCER RESEARCH | 2008年 / 14卷 / 08期

关键词：

D O I：

10.1158/1078-0432.CCR-07-4926

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Purpose: Colonic carcinogenesis deranges growth-regulating epidermal growth factor receptors (EGFR). We previously showed that EGFR signals were up-regulated in human aberrant crypt foci (ACF), putative colon cancer precursors. The azoxymethane model of colon cancer recapitulates many aspects of human colonic tumors. Recent studies indicate that flat dysplastic ACF with increased beta-catenin are tumor precursors in this model. We asked, therefore, if EGFR signals are required for flat clysplastic ACF development and cancer progression. Experimental Design: Rats received azoxymethane or saline, and standard chow or chow supplemented with gefitinib, an EGFR inhibitor, for 44 weeks. EGFR signals were quantified in normal colon, flat ACF, and tumors by computerized analysis of immunostains and Western blots. K-ras mutations were assessed by PCR and mRNA for egfr ligands by quantitative real-time PCR. Results: EGFR inhibition with gefitinib decreased the incidence of flat clysplastic ACF from 66% to 36% and tumors from 71% to 22% (P < 0.05). This inhibitor also reduced the overexpressions of cyclin D1 and Cox-2 in flat ACF. Furthermore, in flat ACF, EGFR blockade decreased the upregulation of c-Jun, Fos13, phosphorylated active signal transducers and activators of transcription 3, and CCAAT/enhancer binding protein-beta, potential regulators of cyclin D1 and Cox-2. In colonic tumors, EGFR blockade significantly decreased angiogenesis, proliferation, and progression while also increasing apoptosis (P < 0.05). Gefitinib also inhibited the activations of extracellular signal - regulated kinase, Src, and AKT pathways in tumors. Conclusions: We have shown for the first time that EGFR promotes the development of flat clysplastic ACF and the progression of malignant colonic tumors. Furthermore, we have mechanistically identified several transcription factors and their targets as EGFR effectors in colonic carcinogenesis.

引用

页码：2253 / 2262

页数：10

共 61 条

[1] Subepithelial myofibroblasts express cyclooxygenase-2 in colorectal tubular adenomas [J].