Gymnemic acids inhibit rabbit glyceraldehyde-3-phosphate dehydrogenase and induce a smearing of its electrophoretic band and dephosphorylation

被引：15

作者：

Izutani, Y

Murai, T

Imoto, T

Ohnishi, M

Oda, M

Ishijima, S ^{[1
]}

机构：

[1] Kyoto Prefectural Univ, Grad Sch Agr, Kyoto 6068522, Japan

[2] Tottori Univ, Fac Med, Dept Physiol, Yonago, Tottori 6838503, Japan

来源：

FEBS LETTERS | 2005年 / 579卷 / 20期

关键词：

gymnemic acids; glyceraldehyde-3-phosphate dehydrogenase; dephosphorylation; gamma-cyclodextrin; rabbit;

D O I：

10.1016/j.febslet.2005.06.070

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Gymnemic acids (GA) inhibited rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity. Binding of GA to GAPDH was observed by surface plasmon resonance measurement. Incubation of GAPDH with GA induced a smearing of the GAPDH band in SDS-PAGE. The GA-induced smearing was diminished by prior incubation of GA with gamma-cyclodextrin or by GA treatment with NAD. GA treatment did not affect the electrophoretic mobility of glucose-6-phosphate isomerase and dehydrogenase. GA treatment diminished the GAPDH band detected by an antibody to phosphoserine, but did not affect the phosphoserine bands of glucose-6-phosphate isomerase and dehydrogenase. These results indicated that GA specifically induced dephosphorylation of GAPDH. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4333 / 4336

页数：4

共 21 条

[1]

PHOSPHORYLATION OF D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE BY CA2+/CALMODULIN-DEPENDENT PROTEIN KINASE-II [J].