Simplified method for producing Paracoccidioides brasiliensis exoantigens for use in immunodiffusion tests

被引：53

作者：

Camargo, ZP ^{[1
]}

Berzaghi, R ^{[1
]}

Amaral, CC ^{[1
]}

Silva, SHM ^{[1
]}

机构：

[1] UNIFESP, Disciplina Biol Celular, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo, Brazil

来源：

MEDICAL MYCOLOGY | 2003年 / 41卷 / 06期

关键词：

exoantigens; gp43; immunodiffusion; Paracoccidioides brasiliensis; paracoccidioidomycosis; serology;

D O I：

10.1080/13693780310001615358

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A simplified method to produce Paracoccidioides brasiliensis exoantigens for immunodiffusion testing is proposed. It uses technology accessible for small laboratories with few resources in Latin America, where paracoccidioidomycosis is endemic. This procedure may replace the more complex procedure, originally proposed by Camargo et al. in 1988, that is currently commonly used. It is based on the production of exoantigen by P brasiliensis isolate B339, a good secretor of the characteristic 43 000-Da glycoprotein gp43.

引用

页码：539 / 542

页数：4