Production, extraction and purification of violacein:: an antibiotic pigment produced by Chromobacterium violaceum

被引：95

作者：

Rettori, D ^{[1
]}

Durán, N ^{[1
]}

机构：

[1] Univ Estadual Campinas, Lab Quim Biol, Inst Quim, BR-13083970 Campinas, SP, Brazil

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1998年 / 14卷 / 05期

关键词：

antibiotic; bioreactor; Chromobacterium violaceum; pigment; violacein;

D O I：

10.1023/A:1008809504504

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A procedure for the production, extraction, and purification of violacein was developed using Chromobacterium violaceum (CCT 3496) cultivated on cotton, in modified 1 litre Roux bottles. A surface tray bioreactor was built to perform these experiments. Violacein was extracted with commercial ethanol, and purified by filtration, Soxhlet extraction, crystallization and high performance liquid chromatography. The violacein was analysed and identified by proton and carbon-13 NMR spectroscopies, thermogravimetric analysis, mass spectrometry, UV-VIS spectroscopy and infrared spectroscopy. It was concluded that the product was highly purified violacein.

引用

页码：685 / 688

页数：4

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