YAC transgenic analysis reveals Wilms' Tumour 1 gene activity in the proliferating coelomic epithelium, developing diaphragm and limb

被引:125
作者
Moore, AW
Schedl, A
McInnes, L
Doyle, M
Hecksher-Sorensen, J
Hastie, ND
机构
[1] Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
[2] Max Delbruck Ctr Mol Med, D-13122 Berlin, Germany
[3] Res Inst Mol Pathol, A-1030 Vienna, Austria
基金
英国惠康基金; 英国医学研究理事会;
关键词
coelomic epithelium; diaphragm; epithelial to mesenchymal cell type transition; septum transversum; hepatic sinusoids; interdigital; mesenchymal to epithelial cell type transition; lacZ; limb metanephros; mouse embryo; nephric cord; podocyte; gene regulation; Rete testis Sertoli; transgenic; Wilms' Tumour I gene; yeast artificial chromosome;
D O I
10.1016/S0925-4773(98)00188-9
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Wilms' Tumour 1 gene (WT1) is required for the con ect development of the urogenital system. To examine its regulation and expression, we created several transgenic mouse lines containing a P-galactosidase reporter driven by the human WT1 promoter. A 5 kb promoter weakly recapitulated a subset of the endogenous Wt1 expression pattern. In contrast, 470 and 280 kb YAC transgenes reproduced the correct pattern with high activity and highlighted new expression sites. Wt1 is expressed in the septum transversum revealing how its mutation causes diaphragmatic defects, Wt1 expression in the limb demarcates a zone between chondrogenic and apoptotic domains. Finally, Wt1 is expressed in mesenchymal cells derived from the coelomic epithelium. Based upon these and further data we discuss a Wt1 role in epithelial tt mesenchymal transitions. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:169 / 184
页数:16
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