Chromatographic determination of the association constant between 8-methoxypsoralen and modified β-cyclodextrin:: protective effect of hydroxypropyl-β-cyclodextrin on 8-methoxypsoralen toxicity in human keratinocytes

被引：7

作者：

Guillaume, Y ^{[1
]}

Andre, C ^{[1
]}

Simon, N ^{[1
]}

Gehin, A ^{[1
]}

Guyon, C ^{[1
]}

Thomassin, A ^{[1
]}

Ismaili, L ^{[1
]}

Aubin, F ^{[1
]}

Nicod, L ^{[1
]}

机构：

[1] Fac Med & Pharm, Equipe Sci Seperat & Biopharmaceut, F-25030 Besancon, France

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 798卷 / 02期

关键词：

association constant; 8-methoxypsoraten; hydroxypropyl-cyclodextrin;

D O I：

10.1016/j.jchromb.2003.09.045

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The retention of 8-methoxypsoralen (8-MOP) on an immobilised hydroxypropyl-p-cyclodextrin (HP-beta-CD) column was analysed in HPLC by the determination of its Langmuir distribution isotherm. A such method was used to confirm the potential drug complexing role of this cyclodextrin. The 8-MOP/HP-beta-CD association constant (K) was equal to 29.5 and 18.7 M-1, respectively, at a temperature equal to 5 and 25degreesC, respectively. These association constant values were used to determine the cytotoxicity profile of human keratinocyte cell line (HaCaT) in relation to the complex concentration. It was showed through these data that HP-beta-CD had a cytoprotective since a reverse effect of HP-P-CD on 8-MOP cytotoxicity was observed. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：217 / 222

页数：6

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