HPLC determination of tranexamic acid in pharmaceutical preparations and blood

被引：22

作者：

Khuhawar, MY ^{[1
]}

Rind, FMA ^{[1
]}

机构：

[1] Univ Sindh, Inst Chem, Singh, Pakistan

来源：

CHROMATOGRAPHIA | 2001年 / 53卷 / 11-12期

关键词：

column liquid chromatography; tranexamic acid; 2-hydroxynaphthaldehyde derivatization; pharmaceutical preparations; blood;

D O I：

10.1007/BF02493025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Tranexamic acid (Tr) reacts with 2-hydroxynaphthaldehyde (HN) in aqueous ethanol at neutral pH in a 1:1 molar ratio to form a tranexamic acid-2-hydroxynaphthaldehyde (Tr-HN) derivative, with enhancement of spectrophotometric sensitivity Tr-HN was eluted from a YMC-ODS column (150 x 4.6 mm id) and separated completely from the derivatizing reagent HN. Tr-HN also was separated from paracetamol and mefanemic acid. Quantitation was at 317 nm. The method was used for the determination of Tr from pharmaceutical preparations and blood samples after 2 - 3 hrs of administration of the drug. A number of additives and amino acids did not affect the determination.

引用

页码：709 / 711

页数：3

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