Identifying and characterizing chemical skin sensitizers without animal testing: Colipa's research and method development program

被引：56

作者：

Aeby, P.

Ashikaga, T. ^{[1
]}

Bessou-Touya, S. ^{[2
]}

Schepky, A. ^{[3
]}

Gerberick, F. ^{[4
]}

Kern, P. ^{[5
]}

Marrec-Fairley, M.

Maxwell, G. ^{[6
]}

Ovigne, J. -M. ^{[7
]}

Sakaguchi, H. ^{[8
]}

Reisinger, K. ^{[9
]}

Tailhardat, M. ^{[10
]}

Martinozzi-Teissier, S. ^{[7
]}

Winkler, P. ^{[11
]}

机构：

[1] Shiseido Res Ctr, Yokohama, Kanagawa, Japan

[2] Pierre Fabre, Castres, France

[3] Beiersdorf, Hamburg, Germany

[4] Procter & Gamble Co, Cincinnati, OH USA

[5] Procter & Gamble Co, Strombeek Bever, Belgium

[6] Unilever, Sharnbrook, Beds, England

[7] LOreal, Aulnay Sous Bois, France

[8] Kao Corp, Mibu, Tochigi, Japan

[9] Henkel AG & Co, KGaA, Dusseldorf, Germany

[10] LVMH Rech, St Jean De Braye, France

[11] Johnson & Johnson, Neuss, Germany

来源：

TOXICOLOGY IN VITRO | 2010年 / 24卷 / 06期

关键词：

Skin sensitization; In vitro; Dendritic cells; Skin metabolism; T cells; Model; DENDRITIC CELL ACTIVATION; GENE-EXPRESSION CHANGES; TEST H-CLAT; IN-VITRO; PEPTIDE REACTIVITY; CONTACT ALLERGENS; LANGERHANS CELLS; EXPOSURE DATA; THP-1; CELLS; COSMETIC PRODUCTS;

D O I：

10.1016/j.tiv.2010.07.005

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The sensitizing potential of chemicals is usually identified and characterized using one of the available animal test methods, such as the mouse local lymph node assay. Due to the increasing public and political concerns regarding the use of animals for the screening of new chemicals, the Colipa Skin Tolerance Task Force collaborates with and/or funds research groups to increase and apply our understanding of the events occurring during the acquisition of skin sensitization. Knowledge gained from this research is used to support the development and evaluation of novel alternative approaches for the identification and characterization of skin sensitizing chemicals. At present one in chemico (direct peptide reactivity assay (DPRA)) and two in vitro test methods (cell based assays (MUSST and h-CLAT)) have been evaluated within Colipa inter-laboratory ring trials and accepted by the European Centre for the Validation of Alternative Methods (ECVAM) for pre-validation. Data from all three test methods will be used to support the development of testing strategy approaches for skin sensitizer potency prediction. The replacement of the need for animal testing for skin sensitization risk assessment is viewed as ultimately achievable and the next couple of years should set the timeline for this milestone. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1465 / 1473

页数：9

共 66 条

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