Nitrogen-dependent regulation of medium-chain length polyhydroxyalkanoate biosynthesis genes in pseudomonads

被引:35
作者
Hoffmann, N [1 ]
Rehm, BHA [1 ]
机构
[1] Massey Univ, Inst Mol Biosci, Palmerston North, New Zealand
关键词
nitrogen; polyhydroxyalkanoate; Pseudomonas; regulation; RpoN;
D O I
10.1007/s10529-004-8353-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Comparative transcriptional analysis of polyhdroxyalkanoate (PHA) biosynthesis genes with wild type strains and mutants, which lack the intact alternative sigma factor gene rpoN, was performed using semi-quantitative RT-PCR. In Pseudomonas putida and Pseudomonas aeruginosa, phaI and phaF were co- transcribed. PhaF was a negative regulator of transcription of PHA synthase gene phaC1 but did not serve as auto-repressor. However, the alternative sigma factor RpoN is suggested as negative regulator of phaF transcription. In P. putida, phaI-phaF transcription is strongly dependent on nitrogen availability and PHA accumulation, whereas phaF transcription is not. These data suggested a differential regulation of phaF and phaIF. The phaC1 gene transcription occurred almost independently by of RpoN or nitrogen availability in both pseudomonads.
引用
收藏
页码:279 / 282
页数:4
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