Expression of MRF4 protein in adult and in regenerating muscles in Xenopus

被引：15

作者：

Becker, C ^{[1
]}

Gaspera, BD ^{[1
]}

Guyot, M ^{[1
]}

Donsez, E ^{[1
]}

Armand, AS ^{[1
]}

Charbonnier, F ^{[1
]}

Launay, T ^{[1
]}

Chanoine, C ^{[1
]}

机构：

[1] Univ Paris 05, Ctr Univ St Peres,LNRS, UMR 7060 CNRS, Lab Biol Dev & Differenciat Musculaire, F-75006 Paris, France

来源：

DEVELOPMENTAL DYNAMICS | 2003年 / 227卷 / 03期

关键词：

MRF4; myogenic regulatory factor; myogenesis; regeneration; Xenopus;

D O I：

10.1002/dvdy.10318

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

In Xenopus, previous studies showed that the transcripts of the myogenic regulatory factor (MRF) MRF4 accumulate during skeletal muscle differentiation, but nothing is known about the accumulation of XMRF4 protein during myogenesis. In this report, an affinity-purified polyclonal antibody against Xenopus MRF4 was developed and used to describe the pattern of expression of this myogenic factor in the adult and in regenerating muscles. From young forming myotubes, XMRF4 protein persistently accumulated in nuclei during the regeneration process and was strongly expressed in nuclei of adult muscles. No selective accumulation of XMRF4 protein was detectable at neuromuscular junctions, but XMRF4 immunoreactivity was observed in sole plate nuclei as well as in extrasynaptic myofiber nuclei. We also report that XMRF4 protein accumulated before the establishment of neuromuscular connections, showing that innervation is not necessary for the appearance of XMRF4 protein during muscle regeneration. (C) 2003 Wiley-Liss, Inc.

引用

页码：445 / 449

页数：5

共 37 条

[1] MRF4 gene expression in Xenopus embryos and aneural myofibers [J].