Membrane localization of the electrogenic cation transporter rOCT1 in rat liver

被引：95

作者：

Meyer-Wentrup, F ^{[1
]}

Karbach, U ^{[1
]}

Gorboulev, V ^{[1
]}

Arndt, P ^{[1
]}

Koepsell, H ^{[1
]}

机构：

[1] Univ Wurzburg, Inst Anat, D-97070 Wurzburg, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 248卷 / 03期

关键词：

D O I：

10.1006/bbrc.1998.9034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The polyspecific cation transporter ROCT1 in the rat was the first identified member of a new protein family with 12 presumed membrane-spanning alpha-helices and two large hydrophilic loops. Previous studies showed that rOCT1 is mainly expressed in liver and mediates electrogenic uptake of small organic cations into cells. Antibodies against partial sequences of rOCT1 were raised and their specificity was verified. Immunohistochemistry with rat liver and western blots with isolated membranes of hepatocytes. Antibody reactions were also performed with intact and permeabilized human embryonic kidney cells that were stably transfected with rOCT1. They showed that the large hydrophilic loop after the first alpha-helix of rOCT1 is located extracellularly, while the C-terminus is located intracellularly. Translational regulation is suggested since the message of rOCT1 was distributed throughout the liver lobuli, whereas rOCT1 was observed only in hepatocytes surrounding the central veins.

引用

页码：673 / 678

页数：6

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