Development and characterization of simple sequence repeat DNA markers for Zelkova serrata

被引：19

作者：

Fukatsu, E

Isoda, K

Hirao, T

Takahashi, M

Watanabe, A ^{[1
]}

机构：

[1] Forest Tree Breeding Ctr, Hitachi, Ibaraki 3191301, Japan

[2] Akita Prefectural Univ, Inst Wood Technol, Akita 0160876, Japan

来源：

MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 02期

关键词：

M13-tailed primer method; microsatellite; population genetics; SSR; Zelkova;

D O I：

10.1111/j.1471-8286.2005.00933.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed 14 microsatellite loci from an enriched genomic DNA library of a broad-leaved deciduous tree, Zelkova serrata. Of 198 clones from the library, 112 contained microsatellite repeat regions. The M13-tailed primer method was used for economy. Sequence-specific primer pairs were designed for 58 of 76 candidate clones. Fourteen of these primer pairs successfully amplified polymorphic single loci among 34 individuals collected from the Kanto breeding region in Japan. The expected heterozygosity for the 14 microsatellite markers ranged from 0.378 to 0.876, suggesting that these will prove valuable for breeding and ecological studies on Z. serrata.

引用

页码：378 / 380

页数：3

共 9 条

[1] M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods [J].