Development of a liquid chromatography method for the determination of linezolid and its application to in vitro and human microdialysis samples

被引：54

作者：

Buerger, C

Joukhadar, C

Muller, M

Kloft, C

机构：

[1] Free Univ Berlin, Inst Pharm, Dept Clin Pharm, D-12169 Berlin, Germany

[2] Univ Vienna, Sch Med, Dept Clin Pharmacol, Vienna, Austria

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 796卷 / 01期

关键词：

microdialysis; linezolid;

D O I：

10.1016/j.jchromb.2003.08.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Linezolid is a new, promising antibacterial agent to treat severe infections. A rapid HPLC assay using UV detection for the determination in microdialysate and human plasma was developed. After sample preparation, using acetonitrile for plasma and water for microdialysate, 20 mul was injected and separated on a RP-18 column. Overall, the assay exhibited good precision and accuracy. The diffusion properties of linezolid investigated in in vitro microdialysis experiments revealed a mean relative recovery of 77.5% (CV: 5.4%; delivery and recovery experiments). Following characterization of linezolid in in vitro microdialysis, the setting is suitable for application in clinical studies. (C) 2003 Elsevier B.V. All rights reserved.

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收藏

页码：155 / 164

页数：10

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