β-Catenin signaling contributes to stemness and regulates early differentiation in murine embryonic stem cells

被引：105

作者：

Anton, Roman ^{[1
]}

Kestler, Hans A. ^{[2
,3
]}

Kuehl, Michael ^{[1
]}

机构：

[1] Univ Ulm, Inst Biochem & Mol Biol, D-89069 Ulm, Germany

[2] Univ Ulm, Univ Hosp Internal Med 1, D-89081 Ulm, Germany

[3] Univ Ulm, Inst Neural Informat Proc, D-89081 Ulm, Germany

来源：

FEBS LETTERS | 2007年 / 581卷 / 27期

关键词：

ES cells; Wnt signaling; stemness; beta-catenin;

D O I：

10.1016/j.febslet.2007.10.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ES cells can self-renew while preserving pluripotency and are able to differentiate into many cell types. In both processes, different signal transduction pathways are implicated, including the Wnt/beta-catenin pathway, which we here further analyzed. We found that a loss of beta-catenin in ES cells leads to altered expression of stem cell marker genes. TCF/beta-catenin reporter gene assays indicate that undifferentiated murine ES cells are capable of reacting to LiCl and Wnt3a but not Wnt5a stimulation, but have low endogenous TCF/beta-catenin activity. Oct-3/4, nanog and Wntl1 were able to repress TCF/beta-catenin transcriptional activity. During differentiation, activation of the Wnt/beta-catenin pathway influences formation of mesoderm and cardiomyocytes in a time and dose dependent manner. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

收藏

页码：5247 / 5254

页数：8

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