Difference in infrared spectra from cultured cells dependent on cell-harvesting method

被引：28

作者：

Fujioka, N

Morimoto, Y

Takeuchi, K

Yoshioka, M

Kikuchi, M

机构：

[1] Natl Def Med Coll, Dept Anat 1, Tokorozawa, Saitama 3598513, Japan

[2] Natl Def Med Coll, Dept Med Engn, Tokorozawa, Saitama 3598513, Japan

来源：

APPLIED SPECTROSCOPY | 2003年 / 57卷 / 02期

关键词：

Fourier transform infrared spectroscopy; human gastric adenocarcinoma cell line; disaggregation; scraping; trypsin; ethylenediaminetetraacetic acid;

D O I：

10.1366/000370203321535187

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

The difference in infrared (IR) spectra may be related to morphological changes caused by the harvesting process itself. On the other hand, the selection of the solution for aggregated cells did not affect the IR spectra. It is believed from the given results that the IR spectra from cells should be obtained with a single harvesting method.

引用

页码：241 / 243

页数：3

共 9 条

[1]

Alberts B., 1997, Molecular Biology of the Cell

[2]

BARRY B, 1992, J RAMAN SPECTROSC, V23, P64

[3]

Freshney R. I, 2010, CULTURE ANIMAL CELLS

[4] BAND ANALYSIS OF HYDRATED HUMAN SKIN STRATUM CORNEUM ATTENUATED TOTAL REFLECTANCE FOURIER TRANSFORM INFRARED SPECTRA IN VIVO [J].