Mice with a fluorescent marker for interleukin 2 gene activation

被引:86
作者
Naramura, M [1 ]
Hu, RJ [1 ]
Gu, H [1 ]
机构
[1] National Institute Allergy & Infectious Diseases, Immunol Lab, NIH, Rockville, MD 20852 USA
基金
日本学术振兴会;
关键词
D O I
10.1016/S1074-7613(00)80603-2
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Production of interleukin (IL)-2 by T lymphocytes is one of the earliest events during immune response. A mutant mouse strain was generated by replacing the IL-2 gene with a cDNA encoding green fluorescent protein (GFP). In this model, GFP fluorescence is readily detectable upon T cell activation and is mostly coexpressed with IL-2 at the single cell level. Thus, individual activated T cells can express the IL-2 gene biallelically. Upon stimulation through the T cell antigen receptor, CD4(+) cells separate into distinct GFP(+) and GFP(-) populations, both of which are capable of differentiating into either Th1 or Th2 effecters. These mice allow noninvasive detection of IL-2 production by single cells and analysis of the subsequent differentiative fate of these cells as an immune response develops.
引用
收藏
页码:209 / 216
页数:8
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