Expression of estrogen receptor alpha and beta in peritoneal and ovarian endometriosis

被引:126
作者
Matsuzaki, S
Murakami, T
Uehara, S
Canis, M
Sasano, H
Okamura, K
机构
[1] Tohoku Univ, Sch Med, Dept Obstet & Gynecol, Aoba Ku, Sendai, Miyagi 9808574, Japan
[2] Polyclin Hotel Dieu, Dept Gynecol, Clermont Ferrand, France
[3] Tohoku Univ, Sch Med, Dept Pathol, Sendai, Miyagi 9808574, Japan
关键词
estrogen receptor isoforms; endometriosis; in situ hybridization; TaqMan reverse transcription-polymerase chain reaction;
D O I
10.1016/S0015-0282(01)01783-6
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To quantify and compare messenger RNA (mRNA) levels of ER-alpha and ER-beta among ovarian endometriotic cysts and red and black peritoneal endometriotic lesions. Design: Prospective study. Setting: University hospital. Patient(s): Patients with or without endometriosis. Intervention(s): Samples of peritoneal (n = 33) and ovarian endometriotic lesions (n = 37) were obtained during laparoscopic surgery. Normal eutopic endometrial tissues and macroscopically normal peritoneal tissues were obtained as controls during or just after surgery. Main Outcome Measure(s): Expression of mRNA for ER-alpha and ER-beta, using a real-time reverse transcription (RT)-PCR assay, TaqMan RT-PCR, and nonradioactive in situ hybridization (ISH) techniques. Result(s): Both eutopic endometrium and endometriotic tissues showed predominantly higher levels of ER-alpha than ER-beta mRNA. Relative ratio of ER-alpha to ER-beta (ER-alpha /ER-beta) mRNA in red peritoneal lesions was significantly higher than in black lesions and ovarian endometriotic cysts. There was no significant difference in ER-alpha /ER-beta between proliferative eutopic endometrium and red peritoneal lesions. These results were confirmed by ISH analysis, which also revealed that the two estrogen receptors were localized in both epithelial and stromal cells of endometriotic tissues. Conclusion(s): The predominant expression of ER-alpha in both glandular epithelial and stromal cells may be essential for the development and growth of peritoneal and ovarian endometriosis. (Fertil Steril(R) 2001;75. 1198-1205. (C) 2001 by American Society for Reproductive Medicine.).
引用
收藏
页码:1198 / 1205
页数:8
相关论文
共 29 条
[1]   Oestrogen receptor (ER)-α and ER-β isoforms in normal endometrial and endometriosis-derived stromal cells [J].
Brandenberger, AW ;
Lebovic, DI ;
Tee, MK ;
Ryan, IP ;
Tseng, JF ;
Jaffe, RB ;
Taylor, RN .
MOLECULAR HUMAN REPRODUCTION, 1999, 5 (07) :651-655
[2]   Tissue distribution of estrogen receptors alpha (ER-alpha) and beta (ER-beta) mRNA in the midgestational human fetus [J].
Brandenberger, AW ;
Tee, MK ;
Lee, JY ;
Chao, V ;
Jaffe, RB .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1997, 82 (10) :3509-3512
[3]  
Canis M, 1997, FERTIL STERIL, V67, P817
[4]   Mechanism of estrogen action:: Lessons from the estrogen receptor-α knockout mouse [J].
Cooke, PS ;
Buchanan, DL ;
Lubahn, DB ;
Cunha, GR .
BIOLOGY OF REPRODUCTION, 1998, 59 (03) :470-475
[5]   Revertant mosaicism:: partial correction of a germ-line mutation in COL17A1 by a frame-restoring mutation [J].
Darling, TN ;
Yee, C ;
Bauer, JW ;
Hintner, H ;
Yancey, KB .
JOURNAL OF CLINICAL INVESTIGATION, 1999, 103 (10) :1371-1377
[6]   Vascular endothelial growth factor (VEGF) in endometriosis [J].
Donnez, J ;
Smoes, P ;
Gillerot, S ;
Casanas-Roux, F ;
Nisolle, M .
HUMAN REPRODUCTION, 1998, 13 (06) :1686-1690
[7]   Immuno-LCM: Laser capture microdissection of immunostained frozen sections for mRNA analysis [J].
Fend, F ;
Emmert-Buck, MR ;
Chuaqui, R ;
Cole, K ;
Lee, J ;
Liotta, LA ;
Raffeld, M .
AMERICAN JOURNAL OF PATHOLOGY, 1999, 154 (01) :61-66
[8]   Expression of oestrogen receptor-α and -β in ovarian endometriomata [J].
Fujimoto, J ;
Hirose, R ;
Sakaguchi, H ;
Tamaya, T .
MOLECULAR HUMAN REPRODUCTION, 1999, 5 (08) :742-747
[9]   A novel method for real time quantitative RT PCR [J].
Gibson, UEM ;
Heid, CA ;
Williams, PM .
GENOME RESEARCH, 1996, 6 (10) :995-1001
[10]   SEQUENCE AND EXPRESSION OF HUMAN ESTROGEN-RECEPTOR COMPLEMENTARY-DNA [J].
GREENE, GL ;
GILNA, P ;
WATERFIELD, M ;
BAKER, A ;
HORT, Y ;
SHINE, J .
SCIENCE, 1986, 231 (4742) :1150-1154