High-level expression of eukaryotic polypeptides from bacterial chromosomes

被引：14

作者：

Olson, P ^{[1
]}

Zhang, Y ^{[1
]}

Olsen, D ^{[1
]}

Owens, A ^{[1
]}

Cohen, P ^{[1
]}

Nguyen, K ^{[1
]}

Ye, JJ ^{[1
]}

Bass, S ^{[1
]}

Mascarenhas, D ^{[1
]}

机构：

[1] Celtrix Pharmaceut Inc, Dept Mol & Cell Biol, Santa Clara, CA 95054 USA

来源：

PROTEIN EXPRESSION AND PURIFICATION | 1998年 / 14卷 / 02期

关键词：

chromosomal; fusion protein; heterologous; bacterial expression; CELF; patents; eukaryotic polypeptides;

D O I：

10.1006/prep.1998.0973

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Attractive economics and short development timelines have often been cited as reasons for using bacteria to express eukaryotic proteins on a commercial scale. Nevertheless, routine techniques for bacterial expression of heterologous proteins are beset by a variety of technical and legal difficulties. In particular, the use of plasmids to express foreign proteins, popular promoter systems, protein fusion partners, and histidine tags and the recovery of proteins from inclusion bodies are affected by a host of issued patents. Chromosomally encoded leaderless fusions (CELF) offer a variety of technical and legal advantages over existing bacterial expression systems. In this study, we show that CELF can be used to produce a wide assortment of eukaryotic proteins at 10-liter fermentation scale. (C) 1998 Academic Press.

引用

页码：160 / 166

页数：7

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